Efficient Doxorubicin Loading to Isolated Dexosomes of Immature JAWSII Cells: Formulated and Characterized as the Bionanomaterial

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dc.authorid243972en_US
dc.authorid180610en_US
dc.contributor.authorMutlu, Esra Cansever
dc.contributor.authorKaya, Özge
dc.contributor.authorWood, Matthew
dc.contributor.authorMager, Imre
dc.contributor.authorTopkara, Kübra
dc.contributor.author., ve diğer.
dc.date.accessioned2020-10-12T14:03:37Z
dc.date.available2020-10-12T14:03:37Z
dc.date.issued2020
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractImmature dendritic cells (IDc), 'dexosomes', are promising natural nanomaterials for cancer diagnose and therapy. Dexosomes were isolated purely from small-scale-up production by using t25-cell-culture flasks. Total RNA was measured as 1.43 +/- 0.33 ng/10 cell. Despite the fact that they possessed a surface that is highly abundant in protein, this did not become a significant effect on the DOX loading amount. Ultrasonication was used for doxorubicin (DOX) loading into the IDc dexosomes. In accordance with the literature, three candidate DOX formulations were designed as IC50 values; dExoIII, 1.8 mu g/mL, dExoII, 1.2 mu g/mL, and dExoI, 0.6 mu g/mL, respectively. Formulations were evaluated by MTT test against highly metastatic A549 (CCL-185; ATTC) cell line. Confocal images of unloaded (naive) were obtained by CellMask(TM)membrane staining before DOX loading. Although, dexosome membranes were highly durable subsequent to ultrasonication, it was observed that dexosomes could not be stable above 70 degrees C during the SEM-image analyses. dExoIII displayed sustained release profile. It was found that dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) results were in good agreement with each other. Zeta potentials of loaded dexosomes have approximately between -15 to -20 mV; and, their sizes are 150 nm even after ultrasonication. IDcJAWSII dexosomes can be able to be utilized as the "BioNanoMaterial" after DOX loading via ultrasonication technique.en_US
dc.identifier.citationMaterials 2020, 13, 3344en_US
dc.identifier.doi10.3390/ma13153344
dc.identifier.issn2470-0029
dc.identifier.pmid32727156en_US
dc.identifier.scopus2-s2.0-85089747076en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/ma13153344
dc.identifier.wosWOS:000559122300001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSciliten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.subjectJAWSIIen_US
dc.subjectExosomeen_US
dc.subjectDoxorubicinen_US
dc.subjectUltrasonicationen_US
dc.subjectA549 Cellsen_US
dc.subjectNatural nanoparticlesen_US
dc.titleEfficient Doxorubicin Loading to Isolated Dexosomes of Immature JAWSII Cells: Formulated and Characterized as the Bionanomaterialen_US
dc.typeArticleen_US

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