Exosome Production, Isolation and Characterization from A549 Epithelial Carcinoma Cells

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dc.authorid180610en_US
dc.authorid264342en_US
dc.contributor.authorMutlu, Esra Cansever
dc.contributor.authorKaya, Özge
dc.contributor.authorYıldırım, Arzu Birinci
dc.contributor.authorÇetinkaya, Ayhan
dc.date.accessioned2021-01-25T12:36:51Z
dc.date.available2021-01-25T12:36:51Z
dc.date.issued2019
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractExosomes are natural nanoparticles that their special features as a natural, homogeneous, nanosized, targeted vesicles (~ 50- 100 nm) have started to be used in the treatment of cancer very recently They have high avidity (many conformational attachment) to attach onto targeted cancer cell surfaces. They are composed of bioactive double-layered lipid layers in which their original nature has the adhesive proteins interacting with the cancer cell membrane easily. In this study, the exosomes of non-small cell lung cancer, A549-epithelial carcinoma cells were investigated for their potential to be the natural or synthetic drug carrier. Firstly, exosomes of A549 cell line were produced using exosome-free media. Immediately after, isolation of their exosomes were performed by using ultracentrifugation procedure. Their SEM image, particle size and zeta potential measurements, exosomal RNA analysis and Protein Content by Bradford assays were performed. Findings (Size: 168 nm; zeta: -16mV) on the properties of A549 cell exosomes proved their potential to be used as the drug carrier for cancer cell therapy.en_US
dc.identifier.citationHacettepe J. Biol. & Chem., 2019, 47 (4), 383-388en_US
dc.identifier.doi10.15671/hjbc.520101
dc.identifier.issn2149-3596
dc.identifier.trdizinid366507en_US
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/366507
dc.identifier.urihttps://doi.org/10.15671/hjbc.520101
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherHacettepe Üniversitesi, Fen Fakültesien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.subjectEkzosomen_US
dc.subjectUltrasantrifüjen_US
dc.subjectA549 hücre hattıen_US
dc.subjectmiRNAen_US
dc.titleExosome Production, Isolation and Characterization from A549 Epithelial Carcinoma Cellsen_US
dc.title.alternativeA549 Epitelyal Karsinom Hücrelerinden Eksozom Üretimi, İzolasyonu ve Karakterizasyonuen_US
dc.typeArticleen_US

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