Inactive Platelet Rich Plasma in Culture Conditions Increases the Proliferation and Decreases the Apoptosis and Senescence of Human Adipose Derived Mesenchymal Stem Cells

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dc.contributor.authorSalkin, Hasan
dc.date.accessioned2024-03-13T10:33:20Z
dc.date.available2024-03-13T10:33:20Z
dc.date.issued2023
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractBACKGROUND/AIMS: The aim of this study was to evaluate the effects of 10% platelet rich plasma (PRP) on the biological activity of human adipose-derived mesenchymal stem cells (hADSCs).MATERIALS AND METHODS: hADSCs were obtained from Erciyes University Gevher Nesibe Genom and Stem Cell Institute. The cells were transferred into plates at a density of 5,000 cells/cm2 in DMEM supplemented with 10% fetal bovine serum. Immunophenotyping was performed by means of primary antibodies selected against CD44, CD90, CD105, CD34, CD45 and CD73 antigens. The experimental groups were composed of 10% PRP treated cells (the study group) and untreated cells (the study group). 2,5-diphenyltetrazolium bromidetest was performed to demonstrate proliferation. Viability and apoptosis were evaluated in the experimental groups on days 1, 3 and 7. Senescent cells were determined by beta-galactosidase staining in the experimental groups on day 7.RESULTS: hADSCs were positive for the immunophenotype of mesenchymal stem cells. The viability and proliferation parameters were statistically significant and higher in the study group at days 1, 3 and 7. Apoptosis and senescence were lower in the 10% PRP group (p<0.05).CONCLUSION: 10% PRP increases proliferation and viability, and prevents the senescence and total apoptosis in hADSCs. In regenerative medical studies, 10% PRP can be used to increase the biological characteristics of hADSCs.en_US
dc.identifier.doi10.4274/cjms.2021.3236
dc.identifier.endpage146en_US
dc.identifier.issn2149-7893
dc.identifier.issn2536-507X
dc.identifier.issue2en_US
dc.identifier.startpage142en_US
dc.identifier.trdizinid1178719en_US
dc.identifier.urihttps://doi.org/10.4274/cjms.2021.3236
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1178719
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3902
dc.identifier.volume8en_US
dc.identifier.wosWOS:000992822000010en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofCyprus Journal Of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPlatelet rich plasmaen_US
dc.subjectadipose-derived mesenchymal stem cellen_US
dc.subjectapoptosisen_US
dc.subjectproliferationen_US
dc.subjectcellular senescenceen_US
dc.titleInactive Platelet Rich Plasma in Culture Conditions Increases the Proliferation and Decreases the Apoptosis and Senescence of Human Adipose Derived Mesenchymal Stem Cellsen_US
dc.typeArticleen_US

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