Inactive Platelet Rich Plasma in Culture Conditions Increases the Proliferation and Decreases the Apoptosis and Senescence of Human Adipose Derived Mesenchymal Stem Cells
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Galenos Publ House
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
BACKGROUND/AIMS: The aim of this study was to evaluate the effects of 10% platelet rich plasma (PRP) on the biological activity of human adipose-derived mesenchymal stem cells (hADSCs).MATERIALS AND METHODS: hADSCs were obtained from Erciyes University Gevher Nesibe Genom and Stem Cell Institute. The cells were transferred into plates at a density of 5,000 cells/cm2 in DMEM supplemented with 10% fetal bovine serum. Immunophenotyping was performed by means of primary antibodies selected against CD44, CD90, CD105, CD34, CD45 and CD73 antigens. The experimental groups were composed of 10% PRP treated cells (the study group) and untreated cells (the study group). 2,5-diphenyltetrazolium bromidetest was performed to demonstrate proliferation. Viability and apoptosis were evaluated in the experimental groups on days 1, 3 and 7. Senescent cells were determined by beta-galactosidase staining in the experimental groups on day 7.RESULTS: hADSCs were positive for the immunophenotype of mesenchymal stem cells. The viability and proliferation parameters were statistically significant and higher in the study group at days 1, 3 and 7. Apoptosis and senescence were lower in the 10% PRP group (p<0.05).CONCLUSION: 10% PRP increases proliferation and viability, and prevents the senescence and total apoptosis in hADSCs. In regenerative medical studies, 10% PRP can be used to increase the biological characteristics of hADSCs.
Açıklama
Anahtar Kelimeler
Platelet rich plasma, adipose-derived mesenchymal stem cell, apoptosis, proliferation, cellular senescence
Kaynak
Cyprus Journal Of Medical Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
8
Sayı
2
