Inactive Platelet Rich Plasma in Culture Conditions Increases the Proliferation and Decreases the Apoptosis and Senescence of Human Adipose Derived Mesenchymal Stem Cells

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Galenos Publ House

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BACKGROUND/AIMS: The aim of this study was to evaluate the effects of 10% platelet rich plasma (PRP) on the biological activity of human adipose-derived mesenchymal stem cells (hADSCs).MATERIALS AND METHODS: hADSCs were obtained from Erciyes University Gevher Nesibe Genom and Stem Cell Institute. The cells were transferred into plates at a density of 5,000 cells/cm2 in DMEM supplemented with 10% fetal bovine serum. Immunophenotyping was performed by means of primary antibodies selected against CD44, CD90, CD105, CD34, CD45 and CD73 antigens. The experimental groups were composed of 10% PRP treated cells (the study group) and untreated cells (the study group). 2,5-diphenyltetrazolium bromidetest was performed to demonstrate proliferation. Viability and apoptosis were evaluated in the experimental groups on days 1, 3 and 7. Senescent cells were determined by beta-galactosidase staining in the experimental groups on day 7.RESULTS: hADSCs were positive for the immunophenotype of mesenchymal stem cells. The viability and proliferation parameters were statistically significant and higher in the study group at days 1, 3 and 7. Apoptosis and senescence were lower in the 10% PRP group (p<0.05).CONCLUSION: 10% PRP increases proliferation and viability, and prevents the senescence and total apoptosis in hADSCs. In regenerative medical studies, 10% PRP can be used to increase the biological characteristics of hADSCs.


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Platelet rich plasma, adipose-derived mesenchymal stem cell, apoptosis, proliferation, cellular senescence


Cyprus Journal Of Medical Sciences

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