Evaluation of Antioxidant Capacity, Anticholinergic and Antidiabetic Activities, and Phenolic Ingredients of Asphodelus aestivus by LC-MS/MS

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Wiley-V C H Verlag Gmbh

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Herein, it was aimed to evaluate three different extracts of the plant Asphodelus aestivus in terms of their antioxidant capacity, total phenolic content, flavonoid profile, and anticholinergic and antidiabetic activity. In addition, the phenolic content of the A. aestivus extracts was determined by liquid chromatography-mass spectrometry/mass spectrometry. The results obtained in the antioxidant studies were checked against butylated hydroxyanisole, butylated hydroxytoluene, Trolox, and alpha-tocopherol antioxidants, which are reference standards. The half-maximal inhibition concentration (IC50) values of A. aestivus for 1,1-diphenyl-2-picryl-hydrazyl and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) removal activity were 245.015-285.851 and 285.818-371.563 mu g/mL, respectively. Then, the reducing impact of A. aestivus extracts was evaluated by the cupric ion (Cu2+), ferric ion (Fe3+), and Fe3+-TPTZ reducing capabilities. Moreover, 0.058, 0.064, and 0.100 mu g of gallic acid equivalent of phenolic and 0.500, 1.212, and 2.074 mu g of quercetin equivalent of flavonoid contents were determined from 1 mg of ethanol, water, and water-ethanol extracts, respectively. For water-ethanol, ethanol, and water extracts of A. aestivus, IC50 values of 0.062 +/- 0.0001, 0.068 +/- 0.0002, and 0.090 +/- 0.0001 mu g/mL against acetylcholinesterase, respectively, were calculated. In addition, against the enzyme alpha-glucosidase IC50 values of 16.376 +/- 0.2216, 18.907 +/- 0.3004, and 24.471 +/- 0.4929 mu g/mL, respectively, were calculated. Extracts showed considerable biological activities thanks to the important molecules they contain.


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Asphodelus aestivus, Antioxidant activity, Phenolic contents, Acetylcholinesterase, alpha-glucosidase


Chemistry & Biodiversity

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