Evaluation Of İn Vitro And İn Vivo İmmunostimulatory Activities Of Poly (Lactic-Co-Glycolic Acid) Nanoparticles Loaded With Soluble And Autoclaved Leishmania İnfantum Antigens: A Novel Vaccine Candidate Against Visceral Leishmaniasis

Basit Öğe Kaydı
dc.authorid174010en_US
dc.authorid199264en_US
dc.authorid302485en_US
dc.authorid174423en_US
dc.contributor.authorAbamor, Emrah Sefik
dc.contributor.authorAllahverdiyev, Adil
dc.contributor.authorTosyalı, Özlem Ayşe
dc.contributor.authorBağırova, Melahat
dc.contributor.authorAcar, Tayfun
dc.contributor.authorMustafaeva, Zeynep
dc.contributor.authorDerman, Serap
dc.date.accessioned2019-12-30T11:22:42Z
dc.date.available2019-12-30T11:22:42Z
dc.date.issued2019
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractObjective: To prepare and characterize poly lactic-co-glycolic acid (PLGA) nanoparticles loaded with soluble leishmanial antigen or autoclaved leishmanial antigen and explore in vitro and in vivo immunogenicity of antigen encapsulated nanoparticles. Methods: Water/oil/water double emulsion technique was employed to synthesize PLGA nanoparticles, and scanning electron microscopy, Fourier transform infrared spectroscopy and Zeta-potential measurements were used to identify the characteristics of nanoparticles. Cytotoxicity of synthetized nanoparticles on J774 macrophage were investigated by MTT assays. To determine the in vitro immunostimulatory efficacies of nanoparticles, griess reaction and ELISA was used to measure the amounts of NO and cytokines. During the in vivo analysis, Balb/c mice were immunized with vaccine formulations, and protective properties of nanoparticles were measured by Leishman Donovan unit in the liver following the infection. Cytokine levels in spleens of mice were determined by ELISA. Results: MTT assay showed that neither soluble leishmanial antigen nor autoclaved leishmanial antigen encapsulated anoparticles showed cytotoxicity against J774 macrophage cells. Contrary to free antigens, both autoclaved leishmanial antigen-nanoparticle and soluble leishmanial antigen-nanoparticle formulations led to a 10 and 16-fold increase in NO amounts by macrophages, respectively. Leishman Donovan unit calculations revealed that soluble leishmanial antigen-nanoparticles and autoclaved leishmanial antigen-nanoparticles yielded 52% and 64% protection against visceral leishmaniasis in mouse models. Besides, in vitro and in vivo tests demonstrated that by increasing IFN-? and IL-12 levels and inhibiting IL-4 and IL10 secretions, autoclaved leishmanial antigen-nanoparticles and soluble leishmanial antigennanoparticles triggered Th1 immune response. Conclusions: Both autoclaved leishmanial antigen-nanoparticles and soluble leishmanial antigen-nanoparticles formulations provide exceptional in vitro and in vivo immunostimulatory activities. Hence, PLGA-based antigen delivery systems are recommended as potential vaccine candidates against visceral leishmaniasisen_US
dc.identifier.citationAsian Pacific Journal of Tropical Medicine 2019; 12(8): 353-364en_US
dc.identifier.doi10.4103/1995-7645.262564
dc.identifier.scopus2-s2.0-85070390842en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.4103/1995-7645.262564
dc.identifier.wosWOS:000479097800003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWolters Kluweren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.subjectVisceral leishmaniasisen_US
dc.subjectVaccineen_US
dc.subjectDeliveryen_US
dc.subjectImmunostimulanten_US
dc.subjectPoly lactic-co-glycolic acid (PLGA)en_US
dc.subjectNanoparticleen_US
dc.titleEvaluation Of İn Vitro And İn Vivo İmmunostimulatory Activities Of Poly (Lactic-Co-Glycolic Acid) Nanoparticles Loaded With Soluble And Autoclaved Leishmania İnfantum Antigens: A Novel Vaccine Candidate Against Visceral Leishmaniasisen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
80EVAL~1.PDF
Boyut:
1.5 MB
Biçim:
Adobe Portable Document Format
Açıklama:
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Mühendislik Mimarlık Fakültesi
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu