Determining Expression of miRNAs That Potentially Regulate STAT5A And 5B in Dasatinib-sensitive K562 Cells

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dc.authorid260725en_US
dc.contributor.authorAktan, Çağdaş
dc.date.accessioned2019-07-19T08:09:00Z
dc.date.available2019-07-19T08:09:00Z
dc.date.issued2017
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractIn the era of tyrosine kinase inhibitors, resistance still constitutes a problem in chronic myeloid leukemia (CML) patients; thus, new pathway-specific inhibitors like miRNAs have become important in the treatment of refractory patients. There are no satisfying data regarding the miRNAs and anti-miRNA treatment targeting STAT5A and 5B. In this study, we first researched the effect of dasatinib on apoptosis in the CML cell line K562. The expressions of miRNAs possibly targeting both STAT5A and 5B were then determined. The down- and upregulation of the miRNAs were compared using the ??CT method. At the last stage of the study, we used a new primer probe in order to validate the results. The level of hsa-miR-940 was decreased 4.4 times and the levels of hsa-miR-527 and hsa-miR-518a-5p were increased 12.1 and 8 times, respectively, in the dasatinib-treated group when compared to the control group. We detected similar results in the validation step. As a conclusion, we determined the expression profiles of miRNAs targeting STAT5A and 5B that had an important role in the pathogenesis of CML. The data obtained could lead to determining new therapeutic targets for CML patients.en_US
dc.identifier.doi10.3906/biy-1705-66
dc.identifier.issn1300-0152
dc.identifier.pmid30814857en_US
dc.identifier.scopus2-s2.0-85038444700en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.trdizinid255579en_US
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/255579
dc.identifier.urihttps://doi.org/10.3906/biy-1705-66
dc.identifier.wosWOS:000418253100008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYtr_TR
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.subjectmiRNA expressionstr_TR
dc.subjectSTAT5Atr_TR
dc.subjectSTAT5Btr_TR
dc.subjectchronic myeloid leukemiatr_TR
dc.subjectdasatinibtr_TR
dc.subjectK562 cellstr_TR
dc.titleDetermining Expression of miRNAs That Potentially Regulate STAT5A And 5B in Dasatinib-sensitive K562 Cellsen_US
dc.typeArticleen_US

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