Detection Of Carbapenem-Resistant Klebsiella Pneumoniae Strains Harboring Carbapenemase, Beta-Lactamase And Quinolone Resistance Genes İn İntensive Care Unit Patients

Basit Öğe Kaydı
dc.authorid243533en_US
dc.authorid107955en_US
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorÜnlü, Özge
dc.date.accessioned2021-01-27T10:21:01Z
dc.date.available2021-01-27T10:21:01Z
dc.date.issued2020
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractAim: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) strains are important nosocomial pathogens worldwide. In this study, we aimed to reveal the antibiotic resistance of clinical CR-Kp strains and determine the presence of KPC, OXA-48, VIM and IMP carbapenemase genes. CTX-M-1, TEM-1, SHV-1 extended-spectrum beta-lactamase (ESBL) genes, qnrA, qnrB, qnrS plasmid-mediated quinolone resistance genes and sul1 and sul2 sulfonamide resistance genes provided molecular epidemiological data. Methods: A total of 175 K. pneumoniae strains were isolated from clinical samples of patients hospitalised in an intensive care unit (ICU) betweent April and October 2017. The strains were identified with conventional methods, with VITEK 2 (BioMerieux, France) and MALDI-TOF MS (Bruker, USA). Antimicrobial susceptibilities were tested using the disc-diffusion method and E-test (BioMerieux, France). Antimicrobial resistance genes were investigated via real-time PCR in strains identified as CR-Kp. Results: High frequencies of blaTEM-1 (86.36%), blaSHV-1 (86.36%), and blaCTX-M-1 (95.45%) genes were found in CR-Kp strains. Morever, all three ESBL genes coexisted in 77.3% of all strains. blaKPC was detected in 12 (54.55%) of the strains, and 4 of them which had an MIC> 16 ?g/mL to imipenem showed blaOXA-48 positivity as well. The qnrS gene determinant (86.36%) had the highest frequency, and strains carrying qnrA showed higher MICs for ciprofloxacin. Conclusion: CR-Kp strains are able to develop different antimicrobial resistance patterns according to regional changes in antimicrobial therapeutic policies. Thus, it is important to monitor the regional molecular epidemiological data for efficient treatment.en_US
dc.identifier.citationGMS Hygiene and Infection Control 2020, Vol. 15en_US
dc.identifier.doi10.3205/dgkh000366
dc.identifier.issn1029-8479
dc.identifier.pmid33299744en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.3205/dgkh000366
dc.identifier.wosWOS:000598118300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherDeutsche Gesellschaft für Krankenhaushygieneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.subjectCarbapenem-resistant Klebsiella pneumoniaeen_US
dc.subjectBeta lactamaseen_US
dc.subjectCarbapenemaseen_US
dc.subjectQuinolone resistanceen_US
dc.titleDetection Of Carbapenem-Resistant Klebsiella Pneumoniae Strains Harboring Carbapenemase, Beta-Lactamase And Quinolone Resistance Genes İn İntensive Care Unit Patientsen_US
dc.title.alternativeNachweis von Carbapenem-resistenten Klebsiella pneumoniae-Stämmen mit Carbapenemase-, Beta-Lactamase- und Chinolon-Resistenzgenen bei Patienten einer Intensivpflegestationen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
11 Detection of carbapenem-resistant Klebsiella pneumoniae.pdf
Boyut:
1.22 MB
Biçim:
Adobe Portable Document Format
Açıklama:
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Tıp Fakültesi
PubMed İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu