Protective effect of ketamine and bone marrow-derived mesenchymal stem cell on ovarian follicular depletion in a rat ischaemia/reperfusion model: An experimental study

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dc.contributor.authorGergin, Ozlem Oz
dc.contributor.authorPehlivan, Sibel Seckin
dc.contributor.authorGonen, Zeynep Burcin
dc.contributor.authorTuran, Isil Tugce
dc.contributor.authorUlger, Menekse
dc.contributor.authorErkan, Ibrahim
dc.contributor.authorYildiz, Karamehmet
dc.date.accessioned2026-01-31T15:08:19Z
dc.date.available2026-01-31T15:08:19Z
dc.date.issued2025
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractThis study investigated the therapeutic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) and ketamine in alleviating ovarian ischemia-reperfusion (I/R) injury in a rat model. Forty-nine female rats were randomly divided into seven groups, each consisting of seven animals: Control, MSC (1 x106 cells via tail vein), I/R, ketamine (10 mg/kg, i.p.), I/R + ketamine, I/R + MSC, and I/R + ketamine + MSC. Biochemical analyses were performed using ELISA to measure malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS), and total oxidant status (TOS). Histological evaluation included histopathological assessment and follicle counting, while the expression levels of TNF-alpha, IL-6, VEGF, and estradiol receptor (ER) were examined using immunohistochemical staining. Apoptotic cell counts were determined by the TUNEL method.I/R injury caused significant follicular degeneration, vascular congestion, edema, hemorrhage, and leukocyte infiltration, which were markedly improved by both MSC and ketamine treatments. The most pronounced improvement was observed in the MSC group. MSC therapy demonstrated strong anti-inflammatory effects by modulating TNF-alpha and IL-6, enhanced antioxidant defense by reducing MDA levels and increasing SOD and CAT activity. It exerted anti-apoptotic properties by decreasing the number of TUNEL-positive cells. In conclusion, BM-MSCs exhibited superior and longer-lasting protective effects compared to ketamine in repairing ovarian tissue damage induced by I/R injury and preserving fertility.
dc.identifier.doi10.1016/j.jri.2025.104793
dc.identifier.issn0165-0378
dc.identifier.issn1872-7603
dc.identifier.pmid41223779
dc.identifier.scopus2-s2.0-105024122723
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org./10.1016/j.jri.2025.104793
dc.identifier.urihttps://hdl.handle.net/20.500.12662/10656
dc.identifier.volume172
dc.identifier.wosWOS:001617818300001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Ireland Ltd
dc.relation.ispartofJournal of Reproductive Immunology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260128
dc.subjectBone marrow-derived stem cells
dc.subjectFollicular degeneration
dc.subjectIschemia-reperfusion
dc.subjectInfertility
dc.subjectKetamine
dc.titleProtective effect of ketamine and bone marrow-derived mesenchymal stem cell on ovarian follicular depletion in a rat ischaemia/reperfusion model: An experimental study
dc.typeArticle

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