Targeting the PANoptosome with 3,4-Methylenedioxy-?-Nitrostyrene, Reduces PANoptosis and Protects the Kidney against Renal Ischemia-Reperfusion Injury

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dc.contributor.authorUysal, Erdal
dc.contributor.authorDokur, Mehmet
dc.contributor.authorKucukdurmaz, Faruk
dc.contributor.authorAltinay, Serdar
dc.contributor.authorPolat, Sait
dc.contributor.authorBatcioglu, Kadir
dc.contributor.authorSezgin, Efe
dc.date.accessioned2024-03-13T10:35:23Z
dc.date.available2024-03-13T10:35:23Z
dc.date.issued2022
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractObjectives: The objectives of this study were a) to investigate the effect of targeting the PANoptosome with 3,4-methylenedioxy-beta-nitrostyrene (MNS) on PANoptosis in the Renal ischemia-reperfussion (RIR) model b) to investigate the kidney protective effect of MNS toward RIR injury. Methods: Thirty-two rats were divided into four groups randomly. The groups were assigned as Control, Sham, DMSO (dimethyl sulfoxide) and MNS groups. The rats in the MNS group were intraperitoneally given 20 mg/kg of MNS 30 minutes before reperfusion. 2% DMSO solvent that dissolves MNS were given to the rats in DMSO group. Left nephrectomy was performed on the rats under anesthesia at the 6th hour after reperfusion. Glutathione peroxidase (GPx), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and 8-Okso-2'-deoksiguanozin (8-OHdG) levels were measured. Immunohistochemical analysis, electron microscopic and histological examinations were carried out in the tissues. Results: Total tubular injury score was lower in the MNS group (p < 0.001). Caspase-3, Gasdermin D and MLK (Mixed Lineage Kinase Domain Like Pseudokinase) expressions were considerably decreased in the MNS group (p < 0.001). Apoptotic index (AI) was found to be low in the MNS group (p < 0.001). CAT and SOD levels were higher in the MNS Group (p = 0.006, p = 0.0004, respectively). GPx, MDA, and 8-OH-dG levels were similar (p > 0.05) in all groups. MNS considerably improved the tissue structure, based on the electron microscopic analysis. Conclusions: Our results suggested that MNS administrated before the reperfusion reduces pyroptosis, apoptosis and necroptosis. These findings suggest that MNS significantly protects the kidney against RIR injury by reducing PANoptosis as a result of specific inhibition of Nod-like receptor pyrin domain-containing 3 (NLRP 3), one of the PANoptosome proteins.en_US
dc.identifier.doi10.1080/08941939.2022.2128117
dc.identifier.endpage1835en_US
dc.identifier.issn0894-1939
dc.identifier.issn1521-0553
dc.identifier.issue11-12en_US
dc.identifier.pmid36170987en_US
dc.identifier.scopus2-s2.0-85139856800en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1824en_US
dc.identifier.urihttps://doi.org/10.1080/08941939.2022.2128117
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4401
dc.identifier.volume35en_US
dc.identifier.wosWOS:000861278200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofJournal Of Investigative Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPANoptosisen_US
dc.subjectPANoptosomeen_US
dc.subject3,4-methylenedioxy-beta-nitrostyreneen_US
dc.subjectischemiaen_US
dc.subjectrenalen_US
dc.titleTargeting the PANoptosome with 3,4-Methylenedioxy-?-Nitrostyrene, Reduces PANoptosis and Protects the Kidney against Renal Ischemia-Reperfusion Injuryen_US
dc.typeArticleen_US

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