Bacteroidetes and Firmicutes levels in gut microbiota and effects of hosts TLR2/TLR4 gene expression levels in adult type 1 diabetes patients in Istanbul, Turkey

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dc.contributor.authorDemirci, Mehmet
dc.contributor.authorTokman, Hrisi Bahar
dc.contributor.authorTaner, Zeynep
dc.contributor.authorKeskin, Fatma Ela
dc.contributor.authorCagatay, Penbe
dc.contributor.authorBakar, Yesim Ozturk
dc.contributor.authorOzyazar, Mucahit
dc.date.accessioned2024-03-13T10:34:58Z
dc.date.available2024-03-13T10:34:58Z
dc.date.issued2020
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractAim: The aim of this study was to determine and compare the levels of both Bacteroidetes and Firmicutes in the gut microbiota and TLR2/TLR4 gene expression in the blood of patients with type 1 diabetes mellitus (T1DM) and healthy individuals. These results may serve as a preliminary assessment to guide future research. Method: Between January and October 2014, stool and blood samples were collected from 53 adult T1DM patients and 53 age- and gender-matched healthy individuals. Bacteroidetes and Firmicutes levels were assessed from stool sample DNA and TLR2 and TLR4 expression levels were analyzed via qPCR using RNA from EDTA blood samples from both patients and healthy controls. Results: The amounts of Bacteroidetes and Firmicutes were statistically significantly higher and lower, respectively, in the T1DM group than in the healthy control group (p < 0.001 and p < 0.001, respectively). In addition, the Firmicutes/Bacteroidetes ratios in patients with T1DM were significantly lower than in healthy controls. The TLR4 and TLR2 gene expression levels in T1DM patients were significantly upregulated and downregulated, respectively, compared to those in the control group. Conclusion: Our data are the first to show a relationship between T1DM and gut microbiota in our country. In addition, our results provide information about the connections between T1DM, gut microbiota, and TLR2 and TLR4 expression. We believe that Bacteroidetes and Firmicutes in the gut microbiota may play a role in the autoimmune process of T1DM and that these findings should be further investigated in the future. (C) 2019 Elsevier Inc All rights reserved.en_US
dc.description.sponsorshipScientific Research Projects Unit of Istanbul University [37749]en_US
dc.description.sponsorshipThis research was supported by Scientific Research Projects Unit of Istanbul University. Project No: 37749.en_US
dc.identifier.doi10.1016/j.jdiacomp.2019.107449
dc.identifier.issn1056-8727
dc.identifier.issn1873-460X
dc.identifier.issue2en_US
dc.identifier.pmid31677982en_US
dc.identifier.scopus2-s2.0-85074431457en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.jdiacomp.2019.107449
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4185
dc.identifier.volume34en_US
dc.identifier.wosWOS:000512832000010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofJournal Of Diabetes And Its Complicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectType 1 diabetesen_US
dc.subjectBacteroidetesen_US
dc.subjectFirmicutesen_US
dc.subjectGut microbiotaen_US
dc.subjectToll-like receptorsen_US
dc.titleBacteroidetes and Firmicutes levels in gut microbiota and effects of hosts TLR2/TLR4 gene expression levels in adult type 1 diabetes patients in Istanbul, Turkeyen_US
dc.typeArticleen_US

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