Effects of TGF-?1 Overexpression on Biological Characteristics of Human Dental Pulp-derived Mesenchymal Stromal Cells

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dc.authorid257699en_US
dc.authorid164410en_US
dc.authorid12634en_US
dc.contributor.authorSalkın, Hasan
dc.contributor.authorGönen, Zeynep Burçin
dc.contributor.authorErgen, Ergül
dc.contributor.authorBahar, Dilek
dc.contributor.authorÇetin, Mustafa
dc.date.accessioned2019-12-29T11:45:11Z
dc.date.available2019-12-29T11:45:11Z
dc.date.issued2019
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractObjective: The aim of our study was to investigate the effect of Transforming growth factor beta-1 (TGF-?1) genetherapy on the surface markers, multilineage differentiation, viability, apoptosis, cell cycle, DNA damage and senescence of human Dental Pulp-derived Mesenchymal Stromal Cells (hDPSC). Methods: hDPSCs were isolated from human teeth, and were cultured with 20% Fetal Bovine Serum (FBS) in minimum essential media-alpha (?-MEM). TGF-?1 gene transfer into hDPSCs was performed by electroporation method after the plasmid was prepared. The transfection efficiency was achieved by using western blot and flow cytometry analyses and GFP transfection. Mesenchymal stem cell (MSC) markers, multilineage differentiation, cell proliferation, apoptosis, cell cycle, DNA damage and cellular senescence assays were performed by comparing the transfected and non-transfected cells. Statistical analyses were performed using GraphPad Prism. Results: Strong expression of TGF-?1 in pCMV-TGF-?1-transfected hDPSCs was detected in flow cytometry analysis. TGF-?1 transfection efficiency was measured as 95%. Western blot analysis showed that TGF-?1 protein levels increased at third and sixth days in pCMV-TGF-?1-transfected hDPSCs. The continuous TGF-?1 overexpression in hDPSCs did not influence the immunophenotype and surface marker expression of MSCs. Our results showed that TGF-?1 increased osteogenic and chondrogenic differentiation, but decreased adipogenic differentiation. Overexpression of TGF-?1 increased the proliferation rate and decreased total apoptosis in hDPSCs (p<0.05). The number of cells at “S” phase was higher with TGF-?1 transfection (p?0.05). Cellular senescence decreased in TGF-?1 transfected group (p?0.05). Conclusions: These results reflect that TGF-?1 has major impact on MSC differentiation. TGF-?1 transfection has positive effect on proliferation, cell cycle, and prevents cellular senescence and apoptosis.en_US
dc.identifier.citationInternational Journal of Stem Cells Vol. 12, No. 1, 2019en_US
dc.identifier.doi10.15283/ijsc18051
dc.identifier.issn2005-5447
dc.identifier.pmid30595006en_US
dc.identifier.scopus2-s2.0-85065514195en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.15283/ijsc18051
dc.identifier.wosWOS:000463853100017en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKorean Society for Stem Cell Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.subjectDental Pulp-derived Mesenchymal Stem Cellsen_US
dc.subjectTGF-β1en_US
dc.subjectProliferationen_US
dc.subjectMultilineage differentiationen_US
dc.subjectGene therapyen_US
dc.titleEffects of TGF-?1 Overexpression on Biological Characteristics of Human Dental Pulp-derived Mesenchymal Stromal Cellsen_US
dc.typeArticleen_US

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