Protective and therapeutic effects of milrinone on acoustic trauma in rat cochlea

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dc.contributor.authorCeylan, Seyit Mehmet
dc.contributor.authorUysal, Erdal
dc.contributor.authorAltinay, Serdar
dc.contributor.authorSezgin, Efe
dc.contributor.authorBilal, Nagihan
dc.contributor.authorPetekkaya, Emine
dc.contributor.authorDokur, Mehmet
dc.date.accessioned2024-03-13T10:30:42Z
dc.date.available2024-03-13T10:30:42Z
dc.date.issued2019
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractObjectiveThe aim of this study was to investigate the potential protective and therapeutic effects of milrinone, a specific phosphodiesterase (PDE) III inhibitor, on acoustic trauma-induced cochlear injury and apoptosis.MethodsA total number of 30 healthy Wistar albino rats were evenly divided into five groups as follows: group 1 was assigned as control group; group 2 and 3 were assigned as low-dosage groups (0.25mg/kg) in which milrinone was administered 1h before acoustic trauma (AT) and 2h after AT, respectively; group 4 and 5 were assigned as high-dosage groups (0.50mg/kg) in which the drug was administered 1h before AT and 2h after AT, respectively. Except control group, all treatment groups received a single dosage of milrinone for 5days. Distortion product otoacoustic emissions (DPOAE) measurements were recorded before AT as well as at second and fifth post-traumatic days. At the end of fifth day, all rats were sacrificed and the cochlea of the rats was removed for histopathological evaluation. In addition, the groups were compared in terms of apoptotic index via caspase-3 staining.ResultsIn terms of signal-to-noise ratio (SNR), there was no statistically significant difference among the groups following AT (p>0.05). After 5days of milrinone treatment, the best SNR values were found in group 5, though all groups did not statistically differ (p>0.05). In histopathological evaluation, vacuolization, inflammation, and edema scores in all treatment groups were statistically lower than those of the control group (p<0.05). In group 2 and 4 where the drug was administered before AT, the inflammation and apoptosis index was lower than those of group 3 and 5 where the drug was administered after AT (p<0.0001).ConclusionWe reveal that milrinone has a protective effect on cochlear damage in the experimental acoustic model of rats. This protective effect was more apparent following the pre-traumatic milrinone administration, and is associated with its effect on decreasing inflammation and apoptosis. Based on DPOAE measurements following AT, especially in the group 5 (high-dosage group), milrinone may also have a therapeutic effect.en_US
dc.identifier.doi10.1007/s00405-019-05417-5
dc.identifier.endpage1931en_US
dc.identifier.issn0937-4477
dc.identifier.issn1434-4726
dc.identifier.issue7en_US
dc.identifier.pmid30955065en_US
dc.identifier.scopus2-s2.0-85064265504en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1921en_US
dc.identifier.urihttps://doi.org/10.1007/s00405-019-05417-5
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3505
dc.identifier.volume276en_US
dc.identifier.wosWOS:000472048600007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofEuropean Archives Of Oto-Rhino-Laryngologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMilrinoneen_US
dc.subjectAcoustic traumaen_US
dc.subjectHearing lossen_US
dc.subjectCaspase 3en_US
dc.subjectApoptosisen_US
dc.subjectInner earen_US
dc.titleProtective and therapeutic effects of milrinone on acoustic trauma in rat cochleaen_US
dc.typeArticleen_US

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