Effects of combination TGF-B1 transfection and platelet rich plasma (PRP) on three-dimension chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells

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dc.contributor.authorSalkin, Hasan
dc.contributor.authorGonen, Zeynep Burcin
dc.contributor.authorOzcan, Servet
dc.contributor.authorBahar, Dilek
dc.contributor.authorLekesizcan, Ayca
dc.contributor.authorTaheri, Serpil
dc.contributor.authorKutuk, Nukhet
dc.date.accessioned2024-03-13T10:35:22Z
dc.date.available2024-03-13T10:35:22Z
dc.date.issued2021
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractAim: The aim of this study was to evaluate the effects of standard culture medium and chondrogenic differentiation medium with PRP on chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cells (rabbit DPSCs) that are transfected with transforming growth factor-beta 1 (TGF-B1) gene, based on the hypothesis of TGF- B1 and PRP can be effective on the chondrogenesis of stem cells. Materials and Methods: Rabbit DPSCs were characterized by using flow cytometry, immunofluorescent staining, quantitative Real Time Polymerase Chain Reaction (qRT-PCR) and differentiation tests. For the characterization, CD29, CD44 and CD45 mesenchymal cell markers were used. Rabbit DPSCs were transfected with TGF-B1 gene using electroporation technique in group 1; with PRP 10% in group 2; with chondrogenic medium in group 3; with both chondrogenic medium and PRP in group 4. DPSCs were cultured in medium with 10% inactive PRP in group 5, chondrogenic medium in group 6, chondrogenic medium with PRP 10% in group 7. SOX9, MMP13 and Aggrecan gene expression levels were evaluated in 3, 6, 12. and 24. days by qRT-PCR. Results: The expression levels of SOX9, MMP13 and Aggrecan were higher in group 2, 3 and group 7 in 3th day however in 24th day group 7 and group 2 were found higher. The expression levels changed by time-dependent. The extracellular matrix of the cells in experimental groups were positively stained with safranin O and toluidine blue. Conclusion: The combination in culture medium of TGF-B1 gene transfection and 10% PRP accelerates the chondrogenic differentiation of DPSCs.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [213S120]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey [213S120].en_US
dc.identifier.doi10.1080/03008207.2019.1675649
dc.identifier.endpage237en_US
dc.identifier.issn0300-8207
dc.identifier.issn1607-8438
dc.identifier.issue2en_US
dc.identifier.pmid31581853en_US
dc.identifier.scopus2-s2.0-85074333566en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage226en_US
dc.identifier.urihttps://doi.org/10.1080/03008207.2019.1675649
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4393
dc.identifier.volume62en_US
dc.identifier.wosWOS:000489798400001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofConnective Tissue Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDental pulp stem cellsen_US
dc.subjectchondrogenic differentiationen_US
dc.subjectTGF-B1en_US
dc.subjectplatelet rich plasma (PRP)en_US
dc.subjectgene transfectionen_US
dc.titleEffects of combination TGF-B1 transfection and platelet rich plasma (PRP) on three-dimension chondrogenic differentiation of rabbit dental pulp-derived mesenchymal stem cellsen_US
dc.typeArticleen_US

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