An Investigation on Yellow (Sinapis alba) and Black (Brassica nigra) Mustard Seed Extracts: Antioxidant, Anticholinergic Activities, and Phenolic Analysis via Liquid Chromatography-Tandem Mass Spectrometry

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dc.contributor.authorPolat Kose, Leyla
dc.date.accessioned2026-01-31T15:08:08Z
dc.date.available2026-01-31T15:08:08Z
dc.date.issued2025
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractIn this study, the biological activities of water and ethanol extracts from Sinapis alba and Brassica nigra seeds were evaluated using various bioanalytical methods and spectrophotometric analyses. Antioxidant capacities were assessed through cupric reducing antioxidant capacity, ferric reducing antioxidant power assay, and ferric ion reducing assays, while radical scavenging abilities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH)center dot and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonate) diammonium salt (ABTS)center dot+ assays. Total phenolic content (TPC) and total flavonoid content (TFC) were quantified as gallic acid equivalents (GAE) and quercetin equivalents (QE), respectively. Additionally, anticholinergic activity was determined by Ellman's method. Qualitative and quantitative analyses of phenolic compounds in extracts were performed using liquid chromatography-tandem mass spectrometry. Reference standards were employed for the comparison. The half-maximal inhibitory concentration (IC50) values of S. alba and B. nigra seed extracts were in the range 1199-1799 mu g/mL for DPPH center dot and 786.6-1259 mu g/mL for ABTS center dot+ radical scavenging activities. TPC levels for S. alba and B. nigra water and ethanol extracts varied between 12.35 and 28.82 mg GAE/g extract, while TFC values ranged from 12.18 to 28.36 mg QE/g extract. The extracts also showed potent acetylcholinesterase inhibitory effects, with IC50 values in the range of 0.167-0.194 mu g/mL. These significant biological activities are attributed to the rich phytochemical composition of the seed extracts, indicating their potential as natural sources of antioxidant and anticholinergic agents.
dc.identifier.doi10.1002/cbdv.202500762
dc.identifier.issn1612-1872
dc.identifier.issn1612-1880
dc.identifier.issue10
dc.identifier.pmid40876013
dc.identifier.scopus2-s2.0-105014617115
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org./10.1002/cbdv.202500762
dc.identifier.urihttps://hdl.handle.net/20.500.12662/10590
dc.identifier.volume22
dc.identifier.wosWOS:001559312800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChemistry & Biodiversity
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260128
dc.subjectanticholinergic activity
dc.subjectantioxidant activity
dc.subjectBrassica nigra
dc.subjectphenolic compounds
dc.subjectSinapis alba
dc.titleAn Investigation on Yellow (Sinapis alba) and Black (Brassica nigra) Mustard Seed Extracts: Antioxidant, Anticholinergic Activities, and Phenolic Analysis via Liquid Chromatography-Tandem Mass Spectrometry
dc.typeArticle

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