Dual-function antimicrobial and SERS-active colloidal silver nanoparticle platform for the detection of opportunistic pathogens assisted by machine learning

Basit Öğe Kaydı
dc.contributor.authorSahin, Furkan
dc.date.accessioned2026-01-31T15:08:12Z
dc.date.available2026-01-31T15:08:12Z
dc.date.issued2026
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractThe rapid control of opportunistic pathogens-through both their detection and elimination-is crucial to mitigate health risks, especially for immunocompromised individuals. In this study, silver nanoparticles (AgNPs) were synthesized via a simple and environmentally friendly approach, using ascorbic acid reduction under medical steam sterilizer conditions, and evaluated as a dual-function colloidal platform that integrates antimicrobial activity and surface-enhanced Raman scattering (SERS)-based pathogen identification. Structural characterization using scanning electron microscopy, zeta potential, UV-Vis spectroscopy, and X-ray diffraction confirmed uniform morphology, high colloidal stability, and crystallinity. The AgNP substrates provided robust and reproducible SERS signals, achieving an analytical enhancement factor of approximately 106 at concentrations down to 1 nM, with sample-to-sample variability below 5%. The antimicrobial assays demonstrated rapid biocidal activity, with > 99% (R > 2) of Escherichia coli, Staphylococcus aureus, and Candida albicans eliminated within 1 h, and significant bactericidal efficacy (R > 6) maintained over 48 h. Importantly, the controlled antimicrobial interaction facilitated the release of intracellular components, yielding more consistent and distinctive spectral profiles that enhanced species-level discrimination. Principal component analysis (PCA) revealed clear separation among pathogen-specific spectral clusters, while a linear support vector machine (SVM) classifier achieved 95.7% accuracy, confirming strong discriminative capability. Overall, this study serves as a proof of concept for a dual-function colloidal AgNP platform, synthesized via an accessible autoclave-assisted green approach, that combines antimicrobial action with SERS-based pathogen identification in a simple and reproducible format.
dc.description.sponsorshipHealth Institutes of Turkiye (TUSEB) [35683]
dc.description.sponsorshipThe author would like to thank Prof. Mustafa Serdar Onses (Erciyes University) for providing access to Raman spectroscopy facilities and for his valuable support. Some schematic elements used in Fig. 1 were created with the assistance of BioRender.com under a trial license. The author would like to express their gratitude to BioRender.com for providing this platform. The author thanks the Health Institutes of Turkiye (TUSEB) for providing microbiological testing resources through project support under project number 35683.
dc.identifier.doi10.1007/s11051-025-06532-7
dc.identifier.issn1388-0764
dc.identifier.issn1572-896X
dc.identifier.issue1
dc.identifier.scopus2-s2.0-105026839848
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org./10.1007/s11051-025-06532-7
dc.identifier.urihttps://hdl.handle.net/20.500.12662/10619
dc.identifier.volume28
dc.identifier.wosWOS:001655010500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Nanoparticle Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260128
dc.subjectSERS
dc.subjectAntimicrobial
dc.subjectSilver nanoparticles
dc.subjectMachine learning
dc.subjectOpportunistic pathogens
dc.subjectNanobiomedicine
dc.titleDual-function antimicrobial and SERS-active colloidal silver nanoparticle platform for the detection of opportunistic pathogens assisted by machine learning
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu