Assessing the efficacy of a novel sperm-washing medium enriched with serotonin, L-carnitine, and coenzyme Q10: an observational cohort study

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dc.contributor.authorDogan, Sinem
dc.contributor.authorAydin, Turgut
dc.contributor.authorKoroglu, Nadiye
dc.contributor.authorYilmazer, Yasemin
dc.contributor.authorAlbayrak, Nazli
dc.contributor.authorCetin, Fadime
dc.contributor.authorMoshfeghi, Elnaz
dc.date.accessioned2025-03-09T10:48:33Z
dc.date.available2025-03-09T10:48:33Z
dc.date.issued2024
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractThis observational cohort study investigated the potential of a novel sperm-washing medium (SWM) enriched with serotonin (5-HT), L-carnitine (L-C), and coenzyme Q10 (CoQ10) to enhance sperm motility and reduce DNA damage. It compared this innovative medium (5-HT/L-C/CoQ10 SWM) with two widely used commercial media (SWM 1 and SWM 2). Ninety-eight volunteers from an infertility clinic provided semen samples, which were divided into three aliquots for analysis in different SWMs: group 1, SWM was composed of hydroxyethyl piperazineethanesulfonic acid (HEPES), sodium bicarbonate, human serum albumin (HSA), taurine, and gentamicin sulfate (SWM 1); group 2, SWM was composed of HEPES, sodium bicarbonate, and HSA (SWM 2); and group 3, SWM was composed of HEPES-buffered human tubal fluid supplemented with 5-HT, L-C, and CoQ10 (5-HT/L-C/CoQ10 SWM). Sperm motility was categorized as progressive, nonprogressive, or immotile. Apoptosis, reactive oxygen species (ROS) production, and DNA fragmentation were also assessed. There were no significant differences in total or progressive sperm motility among the groups. Spermatozoa in group 3 exhibited reduced apoptosis, necrosis, and ROS levels and increased viability. No significant differences were observed in the DNA fragmentation index among groups. The 5-HT/L-C/CoQ10 SWM reduced sperm oxidative stress and apoptosis compared with those of the two commercially available SWMs, suggesting that 5-HT/L-C/CoQ10 SWM could be useful for enhancing in vitro fertilization success rates.
dc.description.sponsorshipTurkish Scientific and Technological Research Council (TUBITAK) [TUBITAK-7218008]; Scientific Research Projects Unit of Istanbul Kultur University [IKU-BAP2212]
dc.description.sponsorshipThis study was financially supported by the Turkish Scientific and Technological Research Council (TUBITAK; No. TUBITAK-7218008), and the Scientific Research Projects Unit of Istanbul Kultur University (No. IKU-BAP2212).
dc.identifier.doi10.4103/aja202425
dc.identifier.endpage639
dc.identifier.issn1008-682X
dc.identifier.issn1745-7262
dc.identifier.issue6
dc.identifier.pmid38856308
dc.identifier.scopus2-s2.0-85199399146
dc.identifier.scopusqualityQ1
dc.identifier.startpage635
dc.identifier.urihttps://doi.org/10.4103/aja202425
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4594
dc.identifier.volume26
dc.identifier.wosWOS:001377332100019
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWolters Kluwer Medknow Publications
dc.relation.ispartofAsian Journal of Andrology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250310
dc.subjectapoptosis
dc.subjectcoenzyme Q10
dc.subjectDNA fragmentation
dc.subjectL-carnitine
dc.subjectserotonin
dc.subjectsperm-washing medium
dc.titleAssessing the efficacy of a novel sperm-washing medium enriched with serotonin, L-carnitine, and coenzyme Q10: an observational cohort study
dc.typeArticle

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