Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology

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dc.contributor.authorYildiz, Asli Pinar Zorba
dc.contributor.authorKoken, Gulnaz Yildirim
dc.contributor.authorAbamor, Emrah Sefik
dc.contributor.authorBagirova, Melahat
dc.contributor.authorTosyali, Ozlem Ayse
dc.contributor.authorKocagoz, Tanil
dc.contributor.authorAllahverdiyev, Adil
dc.date.accessioned2024-03-13T10:33:43Z
dc.date.available2024-03-13T10:33:43Z
dc.date.issued2022
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractBackground: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of diseases such as leishmaniasis. The aim of this study was investigation of the effectiveness of different adjuvants on polyclonal antibody production against L. tropica based on hybridoma technique.Methods: Accordingly, Freund's adjuvant (1956, M. tuberculosis), as a classic adjuvant in studies, was used comparatively with the non-toxic polymeric based Polyoxidonium adjuvant. All animal immunization procedures were conducted at Bezm-i Alem University Experimental Animal Research Center. The adjuvant response was tested both in the serum sample and in the antibodies produced by the hybridomas. The antibody titers were determined with ELISA.Results: Freund's and Polyoxidonium (PO) group blood titer's increased approximately 5.5 fold compared to control after the 6(th) and 8(th) immunization. Hybridomas produced from mice immunized with PO adjuvant induced only antigen-specific antibody response and did not develop an immune response against the adjuvant.Conclusion: Adjuvant selection is very important in terms of the specificity of antibody responses of cells produced in hybridoma technology. Therefore, PO is recommended as a new adjuvant system in this study.en_US
dc.description.sponsorshipRepublic of Turkey, the Ministry of Science, Industry and Technology [2013-2-0254]en_US
dc.description.sponsorshipThe study was supported by the Republic of Turkey, the Ministry of Science, Industry and Technology (Grant No: San-Tez Project 2013-2-0254). In addition, we would like to thank Bezm-i Alem Vakif University Faculty of Medicine Experimental Animals Ethics Committee (Responsible Veterinarian Mert Celikten) , where we conducted our in vivo experiments.en_US
dc.identifier.endpage516en_US
dc.identifier.issn1735-7020
dc.identifier.issn2008-238X
dc.identifier.issue4en_US
dc.identifier.pmid36660415en_US
dc.identifier.scopus2-s2.0-85143289065en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage506en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4130
dc.identifier.volume17en_US
dc.identifier.wosWOS:000900875600007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIranian Scientific Society Medical Entomologyen_US
dc.relation.ispartofIranian Journal Of Parasitologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLeishmaniasisen_US
dc.subjectAntibodyen_US
dc.subjectHybridoma technologyen_US
dc.subjectAdjuvanten_US
dc.subjectVaccineen_US
dc.titlePolymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technologyen_US
dc.typeArticleen_US

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