Screening of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and alpha-Glycosidase Enzyme Inhibition Effects and Antioxidant Activity of Coumestrol

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dc.authorid292052en_US
dc.contributor.authorPolat Köse, Leyla
dc.contributor.authorvd.
dc.date.accessioned2023-03-14T05:31:10Z
dc.date.available2023-03-14T05:31:10Z
dc.date.issued2022
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractCoumestrol (3,9-dihydroxy-6-benzofuran [3,2-c] chromenone) as a phytoestrogen and polyphenolic compound is a member of the Coumestans family and is quite common in plants. In this study, antiglaucoma, antidiabetic, anticholinergic, and antioxidant effects of Coumestrol were evaluated and compared with standards. To determine the antioxidant activity of coumestrol, several methods—namely N,N-dimethyl-p-phenylenediamine dihydrochloride radical (DMPD•+)- scavenging activity, 2,20 -azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS•+ )-scavenging activity, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH• )-scavenging activity, potassium ferric cyanide reduction ability, and cupric ion (Cu2+)-reducing activity—were performed. Butylated hydrox yanisole (BHA), Trolox, ?-Tocopherol, and butylated hydroxytoluene (BHT) were used as the refer ence antioxidants for comparison. Coumestrol scavenged the DPPH radical with an IC50 value of 25.95 µg/mL (r2 : 0.9005) while BHA, BHT, Trolox, and ?-Tocopherol demonstrated IC50 values of 10.10, 25.95, 7.059, and 11.31 µg/mL, respectively. When these results evaluated, Coumestrol had simi lar DPPH• -scavenging effect to BHT and lower better than Trolox, BHA and ?-tocopherol. In addition, the inhibition effects of Coumestrol were tested against the metabolic enzymes acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase II (CA II), and ?-glycosidase, which are associated with some global diseases such as Alzheimer’s disease (AD), glaucoma, and diabetes. Coumestrol exhibited Ki values of 10.25 ± 1.94, 5.99 ± 1.79, 25.41 ± 1.10, and 30.56 ± 3.36 nM towards these enzymes, respectively.en_US
dc.identifier.doi10.3390/molecules27103091
dc.identifier.issn2619-9793
dc.identifier.pmid35630566en_US
dc.identifier.scopus2-s2.0-85130572655en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.3390/molecules27103091
dc.identifier.wosWOS:000802403000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.subjectCoumestrolen_US
dc.subjectCarbonic anhydraseen_US
dc.subjectα-glycosidaseen_US
dc.subjectAcetylcholinesteraseen_US
dc.subjectAntioxidant activityen_US
dc.subjectButyrylcholinesteraseen_US
dc.subjectPhenolic compounden_US
dc.titleScreening of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and alpha-Glycosidase Enzyme Inhibition Effects and Antioxidant Activity of Coumestrolen_US
dc.typeArticleen_US

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