Local application of gingiva-derived mesenchymal stem cells on experimental periodontitis in rats

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dc.contributor.authorBalaban, Yunus Emre
dc.contributor.authorAkbaba, Sema
dc.contributor.authorBozkurt, Serife Buket
dc.contributor.authorBuyuksungur, Arda
dc.contributor.authorAkgun, E. Ece
dc.contributor.authorGonen, Zeynep Burcin
dc.contributor.authorSalkin, Hasan
dc.date.accessioned2024-03-13T10:30:39Z
dc.date.available2024-03-13T10:30:39Z
dc.date.issued2023
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractBackground: Stem cell-based approaches in regenerative periodontal therapy have been used in different experimental models. In this study, the effect of local application of gingival mesenchymal stem cells (GMSC) in fibroin/chitosan oligosaccharide lactate hydrogel (F/COS) on periodontal regeneration was evaluated using experimental periodontitis model in rats.Methods: Mesenchymal stem cells were isolated from the gingiva of rats and characterized. Viability tests and confocal imaging of GMSC in hydrogels were performed. Healthy control without periodontitis (Health; H; n=10), control with periodontitis but no application (Periodontitis; P; n=10), only hydrogel application (F/COS; n=10), and GMSC+F/COS (n=10) four groups were formed for in vivo studies. Experimental periodontitis was created with silk sutures around the maxillary second molars. GMSC labeled with green fluorescent protein (GFP) (250,000 cells/50 mu L) in F/COS were applied to the defect. Animals were sacrificed at 2nd and 8th weeks and maxillae of the animals were evaluated by micro-computed tomography (micro-CT) and histologically. The presence of GFP-labeled GMSC was confirmed at the end of 8 weeks.Results: Micro-CT analysis showed statistically significant new bone formation in the F/COS+GMSC treated group compared with the P group at the end of 8 weeks (p < 0.05). New bone formation was also observed in the F/COS group, but the statistical analysis revealed that this difference was not significant when compared with the P group (p > 0.05). Long junctional epithelium formation was less in the F/COS+GMSC group compared with the P group. Periodontal ligament and connective tissue were well-organized in F/COS+GMSC group.Conclusion: The results showed that local GMSC application in hydrogel contributed to the formation of new periodontal ligament and alveolar bone in rats with experimental periodontitis. Since gingiva is easly accessible tissue, it is promising for autologous cell-based treatments in clinical applications.en_US
dc.description.sponsorshipThis study was supported by the Selcuk University Scientific Research Projects Coordinatorship (Project No: 20132006). The authors do not have any financial interest in the companies whose materials are included in this article. This study was presented or [20132006]; Selcuk University Scientific Research Projects Coordinatorshipen_US
dc.description.sponsorshipThis study was supported by the Selcuk University Scientific Research Projects Coordinatorship (Project No: 20132006). The authors do not have any financial interest in the companies whose materials are included in this article. This study was presented orally at the 51st International Scientific Congress of the Turkish Periodontology Society and received the 1st prize.en_US
dc.identifier.doi10.1002/JPER.23-0246
dc.identifier.issn0022-3492
dc.identifier.issn1943-3670
dc.identifier.pmid37787060en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1002/JPER.23-0246
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3454
dc.identifier.wosWOS:001079559500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Periodontologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjecthydrogelen_US
dc.subjectmesenchymal stem cellen_US
dc.subjectperiodontal regenerationen_US
dc.subjectperiodontitisen_US
dc.titleLocal application of gingiva-derived mesenchymal stem cells on experimental periodontitis in ratsen_US
dc.typeArticleen_US

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