Functional roles of miR-625-5p and miR-874-3p in the progression of castration resistant prostate cancer

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dc.contributor.authorAktan, Cagdas
dc.contributor.authorCal, Cag
dc.contributor.authorKaymaz, Burcin
dc.contributor.authorGunel, Nur Selvi
dc.contributor.authorKipcak, Sezgi
dc.contributor.authorOzel, Buket
dc.contributor.authorGunduz, Cumhur
dc.date.accessioned2024-03-13T10:35:02Z
dc.date.available2024-03-13T10:35:02Z
dc.date.issued2022
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractAims: Androgen receptor (AR) signaling is important in normal prostate and prostate tumor tissues. Thus, the new therapeutic strategies targeting ARs may also be important for treatment of prostate cancer (PC) and its biology. The studies have shown that miRNAs to be dysregulated in PC progression. Therefore, in the present study, differentially expressed miRNAs that predictively target the ARs were identified and investigated by in silico analysis. Main methods: Cellular proliferation, qPCR, western blot and apoptosis assays were performed to investigate the molecular mechanism of the selected miRNAs in the PC cells. Key findings: In our miRNA qPCR study, several miRNAs were found to be differentially regulated in castration resistant prostate cancer (CRPC) cells (LNCaP-Abl and LNCaP-104R2) compared with androgen dependent (AD) cells (LNCaP). The expression levels of miR-625-5p and miR-874-3p were significantly increased in LNCaP-Abl (2.62-fold, p = 0.0002; 4.00-fold, p = 0.00002, respectively) and LNCaP-104R2 (2.44-fold, p = 0.0455; 3.77 fold, p = 0.0383, respectively) compared with AD cells. The expression levels of AR and prostate specific antigen were increased in PC cells compared with AD cells. Furthermore, transfection of PC cells with anti-miRs suppressed their proliferation and AR protein levels (p < 0.05). Significance: Several differentially regulated miRNAs were identified in CRPC cells, including miR-625-5p and miR-874-3p that are potentially involved in PC progression. These results may provide novel insights into the molecular mechanism underlying CRPC cells and miRNA applications may constitute a new and alternative method to prevent development of CRPC cells in the future.en_US
dc.description.sponsorshipEge University Scientific Research Projects Fund [2012/TIP/045]en_US
dc.description.sponsorshipAcknowledgements This study was supported by Ege University Scientific Research Projects Fund which is Grant Number 2012/TIP/045.en_US
dc.identifier.doi10.1016/j.lfs.2022.120603
dc.identifier.issn0024-3205
dc.identifier.issn1879-0631
dc.identifier.pmid35508255en_US
dc.identifier.scopus2-s2.0-85129722804en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.lfs.2022.120603
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4224
dc.identifier.volume301en_US
dc.identifier.wosWOS:000803822700009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofLife Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmicroRNA-625-5pen_US
dc.subjectmicroRNA-874-3pen_US
dc.subjectAndrogen receptoren_US
dc.subjectProstate specific antigenen_US
dc.subjectCastration resistant prostate canceren_US
dc.titleFunctional roles of miR-625-5p and miR-874-3p in the progression of castration resistant prostate canceren_US
dc.typeArticleen_US

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