CHRM3-Associated miRNAs May Play a Role in Bile Acid-Induced Proliferation of H508 Colon Cancer Cells

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dc.contributor.authorAktan, Cagdas
dc.contributor.authorTekin, Fatih
dc.contributor.authorOruc, Nevin
dc.contributor.authorOzutemiz, Omer
dc.date.accessioned2024-03-13T10:33:23Z
dc.date.available2024-03-13T10:33:23Z
dc.date.issued2023
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractBackground: It was well defined that proliferative effects of bile acids on colon epithelium are through interaction with muscarinic-3 receptors. Recently, microRNA emerged as an important regulator of gene expression and has been implicated in pathogenesis of many malignancies. However, the interaction of CHRM3 and microRNAs and their potential effects on colon carcinogenesis remains to be elucidated. Methods: In the current study, analysis of cell proliferation for 6 days after treatment with sodium taurolithocholate was analyzed by using WST-1 method. microRNAs which possibly target CHRM3 were identified by in silico analyses. Expression profiling of these microRNAs, expression changes of CHRM3 gene at mRNA level for H508 and SNU-C4 colon cancer cells were analyzed by quantitative polymerase chain reaction; the protein level of CHRM3 was analyzed using Western blot; apoptotic experiments were analyzed using the Annexin V assay. The Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using the miRPath v3.0. Results: It was found that the expression level of CHRM3 gene was 6.133 +/- 0.698-fold in H508 cells compared with SNU-C4 cells (P =.004). Treatment of H508 cells with sodium taurolithocholate caused 1.34 +/- 0.4156-fold change in the expression level of CHRM3 gene (P =.0448). No apoptotic changes were observed in both colon cancer cells after treatment with sodium taurolithocholate. Different expression changes were detected of hsa-miR-129-5p, hsa-miR-30c-5p, hsa-miR-224-5p, hsa-miR-30b-5p, hsa-miR-522-3p, and hsa-miR-1246. Finally, hsa-miR-1246 and hsa-miR-522-3p could play a critical role in tumor development via bile acid-related genes in colon cancer. Conclusion: These findings reflected that CHRM3-dependent oncogenetic pathways might be in charge of colon cancer. We suggest that the microRNA expression profile of each individual colon cancer tissue is a unique digital signature.en_US
dc.description.sponsorshipTUBITAK (Scientific and Technological Research Council of Turkey) 3001 [114S810]en_US
dc.description.sponsorshipThis study was supported by TUBITAK (Scientific and Technological Research Council of Turkey) 3001 (Grant Number: 114S810).en_US
dc.identifier.doi10.5152/tjg.2022.22605
dc.identifier.endpage307en_US
dc.identifier.issn2148-5607
dc.identifier.issue3en_US
dc.identifier.pmid36919835en_US
dc.identifier.scopus2-s2.0-85150248591
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage298en_US
dc.identifier.trdizinid1169621
dc.identifier.urihttps://doi.org/10.5152/tjg.2022.22605
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3934
dc.identifier.volume34en_US
dc.identifier.wosWOS:001043949300015
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherAvesen_US
dc.relation.ispartofTurkish Journal Of Gastroenterologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjecthsa-miR-1246en_US
dc.subjecthsa-miR-522-3pen_US
dc.subjectcolon canceren_US
dc.subjectbile aciden_US
dc.subjectmuscarinic receptor 3en_US
dc.subjectmicroRNAen_US
dc.titleCHRM3-Associated miRNAs May Play a Role in Bile Acid-Induced Proliferation of H508 Colon Cancer Cellsen_US
dc.typeArticleen_US

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