Nuclear Magnetic Resonance-Based Metabolomic Profiling in Fibromyalgia Patients

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dc.authorid0000-0002-5974-8991
dc.authorid0000-0003-4810-6771
dc.authorid0000-0003-0818-7714
dc.authorid0000-0003-1235-3957
dc.authorid0000-0001-9462-9990
dc.contributor.authorAsik, Hatice Kubra
dc.contributor.authorAtamer, Yildiz
dc.contributor.authorDemirel, Metin
dc.contributor.authorSahbaz, Tugba
dc.contributor.authorSelek, Sahabettin
dc.contributor.authorAlim, Mehtap
dc.contributor.authorAtamer, Aytac
dc.date.accessioned2026-01-31T15:08:09Z
dc.date.available2026-01-31T15:08:09Z
dc.date.issued2025
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractFibromyalgia (FM) is a multifactorial syndrome with poorly understood pathophysiology, characterized by chronic widespread pain and fatigue affecting the central and peripheral nervous systems, as well as the endocrine and muscular systems. These characteristics create significant challenges in diagnosis and treatment. This study compared the metabolic profiles of FM patients and healthy controls using one-dimensional (1D) 1H nuclear magnetic resonance (NMR) spectroscopy and multivariate statistical analysis to identify potential biomarkers associated with FM symptoms. Urine and serum samples from 50 FM patients and 50 healthy individuals underwent untargeted metabolic profiling. Quantitative H-1-NMR spectroscopy was used to determine metabolic changes. Chemometric models and receiver operating characteristic (ROC) curves were generated using the MetaboAnalyst platform. Fold change (FC) analysis and t-tests were conducted to determine statistically significant differences between groups. Our findings showed significant differences at the metabolic level between FM patients and healthy controls. Increased urea, glutamate, valine, taurine, proline, glycine, and homoserine metabolites, and decreased benzoate, leucine, pi-methylhistidine, galactitol, tau-methylhistidine, glutamine, 3-hydroxykynurenine, and fructose levels were observed in FM serum; while increased malate, dimethylamine, trimethylamine N-oxide (TMAO), creatine phosphate, N-phenylacetylglycine, N-acetylglutamate (NAG), hippurate, and urea levels were observed in FM urine, and decreased guanidoacetate, creatine, malonate, serine, glucuronate, creatinine, and uracil levels were observed. Serum glutamate was positively correlated with waist/hip ratio (WHR) and negatively correlated with Fibromyalgia Impact Questionnaire (FIQ) and Visual Analog Scale (VAS); negative correlations existed between taurine and FIQ and VAS; a positive correlation existed between urea and WHR; there was a positive correlation between anserine and body mass index (BMI). Also, in urine, there were positive correlations between TMAO, N-phenylacetylglycine, glutamate, and xylose and WHR; negative correlations existed between glutamine and FIQ; and a positive correlation existed between glucuronate and FIQ. Our findings provide important information about potential biomarkers, associated different metabolites, and metabolic pathways of FM patients; we think that they will provide new insight into the pathogenesis of the disease and help expand our knowledge.
dc.description.sponsorshipBezmialem Vakif University [20250205]; Istanbul Beykent University [2023-24-BAP-09, 2024]
dc.description.sponsorshipWe would like to thank Beykent University Biochemistry Laboratory responsible technician & Idot;brahim Kam & imath;& scedil;, intern doctor & Idot;pek Aydemir, who helped us with the collection and storage of blood and urine samples, Beykent University Purchasing Manager & Scedil;eyma Kurumlar, who helped us with the supply of chemical substances, and Scientific Research Project coordinator Meltem Ery & imath;ld & imath;z for her well-intentioned approach to our research project.
dc.identifier.doi10.1002/nbm.70167
dc.identifier.issn0952-3480
dc.identifier.issn1099-1492
dc.identifier.issue12
dc.identifier.scopus2-s2.0-105019193910
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org./10.1002/nbm.70167
dc.identifier.urihttps://hdl.handle.net/20.500.12662/10600
dc.identifier.volume38
dc.identifier.wosWOS:001619530200013
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofNmr in Biomedicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20260128
dc.subjectmetabolomics
dc.subjectprimer fibromyalgia syndrome
dc.subjectproton nuclear magnetic resonance (H-1-NMR) spectroscopy
dc.titleNuclear Magnetic Resonance-Based Metabolomic Profiling in Fibromyalgia Patients
dc.typeArticle

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