Effects of N-acetylcysteine on sciatic nerve healing: A histopathological, functional, and biochemical study of the rat sciatic nerve

Basit Öğe Kaydı
dc.authoridSELEK, Sahabettin/0000-0003-1235-3957
dc.authoridGunduz, Nermin/0000-0003-1237-5486
dc.authoridYILDIRIM, BURAK/0000-0002-0720-874X
dc.authoridErturk, Cemil/0000-0002-9225-917X
dc.authoridSARITAS, Tahir Burak/0009-0001-3049-1723
dc.authoridBuyukdogan, Halil/0000-0002-0202-444X
dc.contributor.authorSaritas, Tahir Burak
dc.contributor.authorErturk, Cemil
dc.contributor.authorBuyukdogan, Halil
dc.contributor.authorYildirim, Burak
dc.contributor.authorGunduz, Nermin
dc.contributor.authorSelek, Sahabettin
dc.date.accessioned2025-03-09T10:48:32Z
dc.date.available2025-03-09T10:48:32Z
dc.date.issued2024
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractObjectives: This study aims to evaluate the histopathological, biochemical, and functional effects of N-acetylcysteine (NAC), which has antioxidant, anti-inflammatory, and cytoprotective activity, on nerve regeneration in rats with sciatic nerve crush (axonotmesis) injury. Materials and methods: This study used 16 male Wistar rats, which were divided into treatment and control groups. A standard axonotmesis-type surgical injury was induced in the left sciatic nerves of all rats. The treatment group was given 300 mg/kg of intraperitoneal NAC once a day, whereas the control group received an equal volume of saline solution. After conducting gait analyses, the sciatic functional index (SFI) was used for functional assessment. After gait analysis, all animals were euthanized. Blood samples were examined biochemically. The left sciatic nerves and left triceps surae muscles were examined histopathologically. Results: Histopathologically, the thickness of the perineurium, axonal degeneration, axonolysis, edema, inflammation, muscle atrophy, and muscle degeneration were all significantly lower in the treatment group (p<0.05). Functionally, SFI-1, SFI-2, and SFI-3 were significantly higher in the treatment group (p<0.05). Biochemically, while the native thiol level and native thiol/total thiol ratio were significantly higher in the treatment group (p<0.003), the disulfide/total thiol ratio was significantly higher in the control group (p<0.005). Significant correlations were found between six of the seven gait parameters and the histopathological findings (p<0.05). Conclusion: Our study results suggest that NAC may contribute positively to the histopathological and functional recovery of sciatic nerve injury in rats. Furthermore, NAC may have an antioxidant effect on thiol-disulfide homeostasis at a biochemical level. We believe that NAC has a stimulatory effect on healing following nerve injuries.
dc.identifier.doi10.52312/jdrs.2024.1784
dc.identifier.endpage627
dc.identifier.issn2687-4792
dc.identifier.issue3
dc.identifier.pmid39189572
dc.identifier.scopusqualityQ2
dc.identifier.startpage618
dc.identifier.urihttps://doi.org/10.52312/jdrs.2024.1784
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4585
dc.identifier.volume35
dc.identifier.wosWOS:001266037400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Joint Diseases Foundation
dc.relation.ispartofJoint Diseases and Related Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250310
dc.subjectAnimal experiment
dc.subjectantioxidant
dc.subjectN-acetylcysteine
dc.subjectsciatic nerve
dc.subjectthiol-disulfide
dc.titleEffects of N-acetylcysteine on sciatic nerve healing: A histopathological, functional, and biochemical study of the rat sciatic nerve
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu