Problems encountered in conventional HIV 1/2 Algorithms: lack of necessity for immunoblot assays to confirm repeated ELISA reactive results

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dc.contributor.authorYuksel, Pelin
dc.contributor.authorSaribas, Suat
dc.contributor.authorKuskucu, Mert
dc.contributor.authorMutcali, Sibel Islak
dc.contributor.authorKosan, Erdogan
dc.contributor.authorHabip, Zafer
dc.contributor.authorDemirci, Mehmet
dc.date.accessioned2024-03-13T10:33:21Z
dc.date.available2024-03-13T10:33:21Z
dc.date.issued2018
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractBackground: The use of conventional (serologically based) HIV 1/2 diagnostic algorithms has become controversial in recent years. Objectives: Sera from patients who underwent verification tests were evaluated because repeated ELISA-reactive results demonstrated a HIV1+HIV2 positive band pattern. Methods: The line immunoassay (LIA) test was used for repeated HIV enzyme immunoassays (EIA)-reactive sera in patients at three centers. The Bio-Rad Geenius T HIV 1/2 and the HIV-1 RNA tests were used. HIV-1 and RNA HIV-2 were investigated using PCR. Results: LIA was used to evaluate 3,224 out of 10,591 samples with repeated ELISA reactivity (30%). We found that 32 (1%) of the sera, along with HIV1 bands and HIV2 gp36 bands, were positive. Only 28 of the 32 verified serum samples with gp36 bands were repeated, and no gp36 band positivity was detected using the Bio-Rad Geenius T HIV-1/2 confirmatory assay in these serum samples. The HIV-2 proviral DNAs were also negative. Therefore, we excluded the possibility of HIV1+2 co-infection. All samples from the 32 patients were positive for HIV-1 RNA. Conclusion: Our findings highlight the need to exclude confirmatory tests like the LIA test from the current diagnostic HIV algorithm and replace it with rapid HIV-1 and HIV-2 confirmatory immunochromotographic tests.en_US
dc.identifier.doi10.4314/ahs.v18i2.26
dc.identifier.endpage416en_US
dc.identifier.issn1680-6905
dc.identifier.issn1729-0503
dc.identifier.issue2en_US
dc.identifier.pmid30602968en_US
dc.identifier.scopus2-s2.0-85049147084en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage407en_US
dc.identifier.urihttps://doi.org/10.4314/ahs.v18i2.26
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3907
dc.identifier.volume18en_US
dc.identifier.wosWOS:000436373700026en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMakerere Univ, Fac Meden_US
dc.relation.ispartofAfrican Health Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHIVen_US
dc.subjectAIDSen_US
dc.subjectHIV-2en_US
dc.titleProblems encountered in conventional HIV 1/2 Algorithms: lack of necessity for immunoblot assays to confirm repeated ELISA reactive resultsen_US
dc.typeArticleen_US

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