Effects of non-steroidal anti-inflammatory drug (ibuprofen) in low and high dose on stemness and biological characteristics of human dental pulp-derived mesenchymal stem cells

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dc.contributor.authorSalkin, Hasan
dc.contributor.authorBasaran, Kemal Erdem
dc.date.accessioned2024-03-13T10:35:22Z
dc.date.available2024-03-13T10:35:22Z
dc.date.issued2023
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractPurpose The effect of ibuprofen, an NSAID, on biological characteristics such as proliferation, viability, DNA damage and cell cycle in dental pulp derived stem cells (DPSCs) can be important for regenerative medicine. Our aim is to investigate how low and high doses of ibuprofen affect stem cell characteristics in DPSCs. Materials and methods DPSCs were isolated from human teeth and characterized by flow cytometry and differentiation tests. Low dose (0.1 mmol/L) and high dose (3 mmol/L) ibuprofen were administered to DPSCs. Surface markers between groups were analyzed by immunofluorescence staining. Membrane depolarization, DNA damage, viability and cell cycle analysis were performed between groups using biological activity test kits. Cellular proliferation was measured by the MTT and cell count kit. Statistical analyzes were performed using GraphPad Prism software. Results High dose ibuprofen significantly increased CD44 and CD73 expression in DPSCs. High-dose ibuprofen significantly reduced mitochondrial membrane depolarization in DPSCs. It was determined that DNA damage in DPSCs decreased significantly with high dose ibuprofen. Parallel to this, cell viability increased significantly in the ibuprofen applied groups. High-dose ibuprofen was found to increase mitotic activity in DPSCs. Proliferation in DPSCs increased in parallel with the increase in mitosis stage because of high-dose ibuprofen administration compared to the control and low-dose ibuprofen groups. Our proliferation findings appeared to support cell cycle analyses. Conclusion High dose ibuprofen improved the immunophenotypes and biological activities of DPSCs. The combination of ibuprofen in the use of DPSCs in regenerative medicine can make stem cell therapy more effective.en_US
dc.identifier.doi10.1080/03008207.2022.2083613
dc.identifier.endpage25en_US
dc.identifier.issn0300-8207
dc.identifier.issn1607-8438
dc.identifier.issue1en_US
dc.identifier.pmid35647871en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage14en_US
dc.identifier.urihttps://doi.org/10.1080/03008207.2022.2083613
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4394
dc.identifier.volume64en_US
dc.identifier.wosWOS:000804643700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofConnective Tissue Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDental pulp-derived stem cellen_US
dc.subjectibuprofenen_US
dc.subjectnon-steroidal anti-inflammatory drugsen_US
dc.subjectproliferationen_US
dc.subjectDNA damageen_US
dc.titleEffects of non-steroidal anti-inflammatory drug (ibuprofen) in low and high dose on stemness and biological characteristics of human dental pulp-derived mesenchymal stem cellsen_US
dc.typeArticleen_US

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