Salmonella Spp. and Shigella Spp. detection via multiplex real-time PCR and discrimination via MALDI-TOF MS in different animal raw milk samples

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dc.contributor.authorDemirci, M.
dc.contributor.authorYigin, A.
dc.contributor.authorAltun, S. K.
dc.contributor.authorUysal, H. K.
dc.contributor.authorSaribas, S.
dc.contributor.authorKocazeybek, B. S.
dc.date.accessioned2024-03-13T10:33:20Z
dc.date.available2024-03-13T10:33:20Z
dc.date.issued2019
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractAims: The aim of this study was to provide epidemiological data about the presence of Salmonella spp. and Shigella spp. in raw milk samples collected from different animals. Methods: A total of 231 raw milk samples from 48 cows, 65 goats, 65 sheep, and 53 donkeys were studied. The ISO 6579:2002 and ISO 21567:2004 methods, antimicrobial susceptibility tests, and serotyping were performed. Species and subspecies discriminations were made via matrix-assisted laser desorption/ionization-time of flight mass spectrometry. After DNA isolation from all samples, Salmonella spp. and Shigella spp. were detected using real-time polymerase chain reaction (PCR) kits. Results: Five samples (2.16%) showed positivity out of 231 raw milk samples for Salmonella spp., and 2 (0.87%) samples were detected to be positive by multiplex real-time PCR design. Conclusion: We found that raw milk samples were not free of Salmonella spp. and Shigella spp. and need to be tested routinely to avoid public health problems. Rapid and reliable real-time PCR method can be developed and used for this purposes instead of slow bacterial culture processes.en_US
dc.identifier.doi10.4103/njcp.njcp_596_18
dc.identifier.endpage1090en_US
dc.identifier.issn1119-3077
dc.identifier.issue8en_US
dc.identifier.pmid31417051en_US
dc.identifier.scopus2-s2.0-85070952208en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1083en_US
dc.identifier.urihttps://doi.org/10.4103/njcp.njcp_596_18
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3896
dc.identifier.volume22en_US
dc.identifier.wosWOS:000481990500008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofNigerian Journal Of Clinical Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectqPCRen_US
dc.subjectSalmonella sppen_US
dc.subjectShigella sppen_US
dc.subjectsubspecies discriminationen_US
dc.titleSalmonella Spp. and Shigella Spp. detection via multiplex real-time PCR and discrimination via MALDI-TOF MS in different animal raw milk samplesen_US
dc.typeArticleen_US

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