Short and long-term blockades of adenosine 2A, 5-HT2A, and 5-HT7 receptors induce apoptosis, reduce proliferation, and show differential effects on miR-27b-3p expression in neuroblastoma cell lines

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dc.authoridKorkmaz, Seyda/0000-0003-1175-9787
dc.contributor.authorBasaran, Kemal Erdem
dc.contributor.authorKorkmaz, Seyda
dc.contributor.authorSatir-Basaran, Guzide
dc.contributor.authorSalkin, Hasan
dc.date.accessioned2025-03-09T10:49:02Z
dc.date.available2025-03-09T10:49:02Z
dc.date.issued2024
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractThe first of our aims in this study was to investigate the effects of 5-HT2AR, 5-HT7R, and A2AR blockades on miR-27b-3p expression in the short and long-term in neuroblastoma cells. Our second aim was to reduce the expression of pERK and suppress proliferation by blocking the 5-HT2AR with ketanserin. Our third aim was to reduce the expression of pAKT and induce apoptosis by blocking the A2AR and 5-HT7R with MSX3 and SB269970. Thus, we aimed to investigate the therapeutic efficacy of ketanserin, MSX3 and SB269970, individually or in combination, on neuroblastoma cells. We found that short and long-term blockades of A2A, 5-HT2A, and 5-HT7 receptors had different effects on miR-27b-3p expression. Blockade of A2AR and 5-HT7R with MSX3 and SB269970 decreased miR-27b-3p expression in the short term while increasing it in the long term. Ketanserin increased miR-27b-3p expression in both the short and long term. When 5-HT2AR was blocked with ketanserin, no significant difference was observed in pERK expression and proliferation in the short term. In contrast, a substantial decrease in pERK expression and proliferation was detected in the long term. Our findings show that the MSX3 + SB269970 dual combination and ketanserin + MSX3 + SB269970 triple combination are especially critical in suppressing pAKT expression in the long term. These findings showed that pAKT protein expression induced apoptosis due to decreased in neuroblastoma cells. Our study provides the first evidence for the relationships between ketanserin/miR-27b-3p/pERK, MSX3/miR27b-3p/pAKT, and SB269970/miR-27b-3p/pAKT in neuroblastoma cells. Ketanserin, MSX3, and SB269970 drug combinations may be promising therapeutic agents in neuroblastoma cells.
dc.description.sponsorshipErciyes University, Betul Ziya Eren Genome and Stem Cell Center (GENKOK)
dc.description.sponsorshipThe authors would like to thank Erciyes University, Betul Ziya Eren Genome and Stem Cell Center (GENKOK) , Kayseri-Turkey because of the facilities they provided.
dc.identifier.doi10.1016/j.neuroscience.2024.11.032
dc.identifier.endpage221
dc.identifier.issn0306-4522
dc.identifier.issn1873-7544
dc.identifier.pmid39547336
dc.identifier.scopus2-s2.0-85209147215
dc.identifier.scopusqualityQ2
dc.identifier.startpage212
dc.identifier.urihttps://doi.org/10.1016/j.neuroscience.2024.11.032
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4700
dc.identifier.volume563
dc.identifier.wosWOS:001360056800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofNeuroscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250310
dc.subjectmiR-27b-3p
dc.subjectKetanserin
dc.subjectMSX3
dc.subjectSB269970
dc.subjectNeuroblastoma
dc.subjectA2A receptor
dc.subjectSerotonin receptor
dc.titleShort and long-term blockades of adenosine 2A, 5-HT2A, and 5-HT7 receptors induce apoptosis, reduce proliferation, and show differential effects on miR-27b-3p expression in neuroblastoma cell lines
dc.typeArticle

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