Comparison of new and classical point mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from dyspeptic patients and their effects on phenotypic clarithromycin resistance

Basit Öğe Kaydı
dc.contributor.authorKocazeybek, Bekir
dc.contributor.authorSakli, Merve Kutlu
dc.contributor.authorYuksel, Pelin
dc.contributor.authorDemirci, Mehmet
dc.contributor.authorCaliskan, Reyhan
dc.contributor.authorSarp, Tevhide Ziver
dc.contributor.authorSaribas, Suat
dc.date.accessioned2024-03-13T10:35:34Z
dc.date.available2024-03-13T10:35:34Z
dc.date.issued2019
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractPurpose. We aimed to investigate the presence of three recently identified point mutations (A2115G, G2141A and A2144T) of the 23 S rRNA gene and compare them with the three most frequently encountered point mutations (A2142G, A2142C and A2143G) in Helicobacter pylori strains in Turkey. Methodology. A total of 63 patients (mean 47.08 +/- 12.27) were included. The E-test method (for clarithromycin) was used for the clarithromycin antimicrobial susceptibility test of isolated H. pylori strains. Real-time PCR was used to detect the point mutations. Results. A total of 24 out of 63 H. pylori strains (38.1%) were detected as clarithromycin resistant (>0.5 mg l(-1)). The new A2115G (n:6, 25%), A2144T (n: 7, 29.1%) and G2141A, 8 (n: 8, 33.3%) mutations and the classical A2142G (n: 8, 33.3%) and A2143G (n: 11, 45.8%) point mutations were detected in the 24 clarithromycin-resistant strains. The A2144T point mutation had the highest median MIC value (3 mg l(-1)) amongst the new mutations, but the classical mutations (A2142G and A2143G) had the highest median MIC values (256 mg l(-1)) overall. The presence of the A2115G (OR: 31.66), A2144T (OR: 36.92) or G2141A (OR: 28.16) mutations increased the likelihood of clarithromycin resistance in H. pylori strains by 31.66-, 36.92- and 28.16-fold (ORs), respectively, according to the binary logistic regression analysis. Conclusion. We concluded that classical mutations of the 23 S rRNA gene resulted in higher clarithromycin MIC values than new mutations. These new point mutations caused moderate elevations in the MIC values of clarithromycin-resistant H. pylori strains.en_US
dc.description.sponsorshipIstanbul University Research Fund [20 900]en_US
dc.description.sponsorshipThis work was supported by the Istanbul University Research Fund under project number 20 900. We are grateful for this support.en_US
dc.identifier.doi10.1099/jmm.0.000944
dc.identifier.endpage573en_US
dc.identifier.issn0022-2615
dc.identifier.issn1473-5644
dc.identifier.issue4en_US
dc.identifier.pmid30724729en_US
dc.identifier.scopus2-s2.0-85064477458en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage566en_US
dc.identifier.urihttps://doi.org/10.1099/jmm.0.000944
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4494
dc.identifier.volume68en_US
dc.identifier.wosWOS:000470084200007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMicrobiology Socen_US
dc.relation.ispartofJournal Of Medical Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHelicobacter pylorien_US
dc.subjectA2115Gen_US
dc.subjectA2144T and G2141Aen_US
dc.subjectpoint mutationsen_US
dc.subjectclarithromycinen_US
dc.titleComparison of new and classical point mutations associated with clarithromycin resistance in Helicobacter pylori strains isolated from dyspeptic patients and their effects on phenotypic clarithromycin resistanceen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu