Polymeric Approach to Adjuvant System in Antibody Production against Leishmaniasis Based on Hybridoma Technology
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Tarih
2022
Yazarlar
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Dergi ISSN
Cilt Başlığı
Yayıncı
IRANIAN SCIENTIFIC SOCIETY MEDICAL ENTOMOLOGY
Erişim Hakkı
Özet
Background: Leishmaniasis is a zoonotic disease, which is one of the serious public health problems in the world. Nowadays, antibody production using hybridoma technology may be a correct approach in terms of sensitivity in the diagnosis of dis eases such as leishmaniasis. The aim of this study was investigation of the effective ness of different adjuvants on polyclonal antibody production against L. tropica based on hybridoma technique. Methods: Accordingly, Freund's adjuvant (1956, M. tuberculosis), as a classic adjuvant in studies, was used comparatively with the non-toxic polymeric based Polyoxidoni um adjuvant. All animal immunization procedures were conducted at Bezm-i Alem University Experimental Animal Research Center. The adjuvant response was tested both in the serum sample and in the antibodies produced by the hybridomas. The antibody titers were determined with ELISA. Results: Freund’s and Polyoxidonium (PO) group blood titer’s increased approxi mately 5.5 fold compared to control after the 6th and 8 th immunization. Hybridomas produced from mice immunized with PO adjuvant induced only antigen-specific antibody response and did not develop an immune response against the adjuvant. Conclusion: Adjuvant selection is very important in terms of the specificity of anti body responses of cells produced in hybridoma technology. Therefore, PO is rec ommended as a new adjuvant system in this study
Açıklama
Anahtar Kelimeler
Leishmaniasis, Antibody, Hybridoma technology, Adjuvant, Vaccine
Kaynak
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