Recombinant plasmid-based quantitative Real-Time PCR analysis of Salmonella enterica serotypes and its application to milk samples

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dc.contributor.authorGokduman, Kurtulus
dc.contributor.authorAvsaroglu, M. Dilek
dc.contributor.authorCakiris, Aris
dc.contributor.authorUstek, Duran
dc.contributor.authorGurakan, G. Candan
dc.date.accessioned2024-03-13T10:35:03Z
dc.date.available2024-03-13T10:35:03Z
dc.date.issued2016
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractThe aim of the current study was to develop, a new, rapid, sensitive and quantitative Salmonella detection method using a Real-Time PCR technique based on an inexpensive, easy to produce, convenient and standardized recombinant plasmid positive control. To achieve this, two recombinant plasmids were constructed as reference molecules by cloning the two most commonly used Salmonella-specific target gene regions, invA and ttrRSBC The more rapid detection enabled by the developed method (21 h) compared to the traditional culture method (90 h) allows the quantitative evaluation of Salmonella (quantification limits of 10(1) CFU/ml and 10(0) CFU/ml for the invA target and the ttrRSBC target, respectively), as illustrated using milk samples. Three advantages illustrated by the current study demonstrate the potential of the newly developed method to be used in routine analyses in the medical, veterinary, food and water/environmental sectors: I - The method provides fast analyses including the simultaneous detection and determination of correct pathogen counts; II - The method is applicable to challenging samples, such as milk; III - The method's positive controls (recombinant plasmids) are reproducible in large quantities without the need to construct new calibration curves. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipMiddle East Technical University (METU) [BAP-03-14-2010-05]en_US
dc.description.sponsorshipThis study was supported by Middle East Technical University (METU) research fund: BAP-03-14-2010-05.en_US
dc.identifier.doi10.1016/j.mimet.2016.01.008
dc.identifier.endpage58en_US
dc.identifier.issn0167-7012
dc.identifier.issn1872-8359
dc.identifier.pmid26820062en_US
dc.identifier.scopus2-s2.0-84957110684en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage50en_US
dc.identifier.urihttps://doi.org/10.1016/j.mimet.2016.01.008
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4231
dc.identifier.volume122en_US
dc.identifier.wosWOS:000371368200011en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Microbiological Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSalmonellaen_US
dc.subjectinvAen_US
dc.subjectttrRSBCen_US
dc.subjectRecombinant plasmidsen_US
dc.subjectReal-Time PCRen_US
dc.subjectMilken_US
dc.titleRecombinant plasmid-based quantitative Real-Time PCR analysis of Salmonella enterica serotypes and its application to milk samplesen_US
dc.typeArticleen_US

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