Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue, and Dental Pulp as Sources of Cell Therapy for Zone of Stasis Burns

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dc.contributor.authorAbbas, Ozan Luay
dc.contributor.authorOzatik, Orhan
dc.contributor.authorGonen, Zeynep Burcin
dc.contributor.authorOgut, Serdal
dc.contributor.authorOzatik, Fikriye Yasemin
dc.contributor.authorSalkin, Hasan
dc.contributor.authorMusmul, Ahmet
dc.date.accessioned2024-03-13T10:35:23Z
dc.date.available2024-03-13T10:35:23Z
dc.date.issued2019
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractIntroduction: The implantation of mesenchymal stem cells (MSCs) has been shown to exert benefits for the survival of the zone-of-stasis. However, the clinical experience indicates the importance of selecting the right source and type of stem cells. Therefore, we planned the current study to perform a quantitative comparison of MSCs isolated from three different sources to provide information useful in selection of the optimal source and to see whether critical mechanisms are conserved between different populations. Methods: The protective effects of MSCs derived from bone marrow, adipose tissue and dental pulp were compared in a rat model of thermal trauma. The stasis zones were evaluated 72 h after the burn using histochemistry, immunohistochemistry and biochemistry. Results: Gross evaluation of burn wounds revealed that the differences between the mean percentages of the calculated necrotic areas weren't statistically significant. Semi-quantitative grading of the histopathological findings revealed that there were no significant differences between damage scores. Immunohistochemical assessment of apoptotic and necrotic cell deaths revealed that the differences between the mean numbers of apoptotic and necrotic cells weren't statistically significant. Myeloperoxidase activity was found to be significantly lower in the adipose tissue group. Biochemical and immunohistochemical assessment of tissue malondialdehyde revealed that the differences between the groups weren't statistically significant. Finally, the number of neo-vessels in the dental pulp group was found to be significantly higher. Conclusion: Our findings suggest that bone marrow, adipose tissue and dental pulp may serve as a universal donor MSC source for the prevention of burn wound progression.en_US
dc.description.sponsorshipAhi Evran University Research Funden_US
dc.description.sponsorshipThis study was approved by the Ethical Committee for Experimental Research on Animals and supported by Ahi Evran University Research Fund. The authors have no financial interest or other relationship with the manufacturers of any products or providers of any service mentioned in this article.en_US
dc.identifier.doi10.1080/08941939.2018.1433254
dc.identifier.endpage490en_US
dc.identifier.issn0894-1939
dc.identifier.issn1521-0553
dc.identifier.issue6en_US
dc.identifier.pmid29442525en_US
dc.identifier.scopus2-s2.0-85042108732en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage477en_US
dc.identifier.urihttps://doi.org/10.1080/08941939.2018.1433254
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4400
dc.identifier.volume32en_US
dc.identifier.wosWOS:000534625200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofJournal Of Investigative Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBurnen_US
dc.subjectstasis zoneen_US
dc.subjectmesenchymal stem cellen_US
dc.subjectbone marrowen_US
dc.subjectdental pulpen_US
dc.subjectadipose tissueen_US
dc.titleComparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Adipose Tissue, and Dental Pulp as Sources of Cell Therapy for Zone of Stasis Burnsen_US
dc.typeArticleen_US

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