Investigating the cryoprotective efficacy of fructans in mammalian cell systems via a structure-functional perspective

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dc.contributor.authorTornaci, Selay
dc.contributor.authorErginer, Merve
dc.contributor.authorGokalsin, Baris
dc.contributor.authorAysan, Arzu
dc.contributor.authorCetin, Metin
dc.contributor.authorSadauki, Mubarak
dc.contributor.authorFindikli, Necati
dc.date.accessioned2024-03-13T10:30:57Z
dc.date.available2024-03-13T10:30:57Z
dc.date.issued2024
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractFructans have long been known with their role in protecting organisms against various stress factors due to their ability to induce controlled dehydration and support membrane stability. Considering the vital importance of such features in cryo-technologies, this study aimed to explore the cryoprotective efficacy of fructans in mammalian cell systems where structurally different fructan polymers were examined on in vitro cell models derived from organs such as the liver, frequently used in transplantation, osteoblast, and cord cells, commonly employed in cell banking, as well as human seminal fluids that are of vital importance in assisted reproductive technology. To gain insights into the fructan/membrane interplay, structural differences were linked to rheological properties as well as to lipid membrane interactions where both fluorescein leakage from unilamellar liposomes and membrane integrity of osteoblast cells were monitored. High survival rates obtained with human endothelial, osteoblast and liver cells for up to two months clearly showed that fructans could be considered as effective non-permeating cryoprotectants, especially for extended periods of cryopreservation. In trials with human seminal fluid, short chained levan in combination with human serum albumin and glycerol proved very effective in preserving semen samples across multiple patients without any morphological abnormalities.en_US
dc.description.sponsorshipTUBITAK [118M607]en_US
dc.description.sponsorshipAcknowledgements This project was founded by TUBITAK 118M607 project for financial support. Authors thank Dr. Demet Sezgin Mansurogylu for her technical assistance in conducting SEM and STEM analysis studies.en_US
dc.identifier.doi10.1016/j.carbpol.2023.121704
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.pmid38220340en_US
dc.identifier.scopus2-s2.0-85180373086en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2023.121704
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3621
dc.identifier.volume328en_US
dc.identifier.wosWOS:001142732400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofCarbohydrate Polymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryopreservationen_US
dc.subjectCryoprotectanten_US
dc.subjectFructanen_US
dc.subjectLevanen_US
dc.subjectInulinen_US
dc.titleInvestigating the cryoprotective efficacy of fructans in mammalian cell systems via a structure-functional perspectiveen_US
dc.typeArticleen_US

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