Diagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infection

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dc.contributor.authorDemirci, Mehmet
dc.contributor.authorSaribas, Suat
dc.contributor.authorOzer, Nigar
dc.contributor.authorToprak, Sezer
dc.contributor.authorCaglar, Emel
dc.contributor.authorOrtakoylu, Gonenc
dc.contributor.authorYuksel, Pelin
dc.date.accessioned2024-03-13T10:34:59Z
dc.date.available2024-03-13T10:34:59Z
dc.date.issued2018
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractBackground: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TagMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard. Methods: 60 patients with confirmed pulmonary TB and 60 individuals without TB were included as the study and control groups, respectively. Sputum specimens were cultured using LJ and BACTEC MGIT 960 systems. Extracted DNA was used for COBAS PCR in a CONAS TagMan 48 analyzer. 85B mRNA detection was performed by RT-qPCR. Results: The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of COBAS TagMan MTB Test were detected as 93.3%, 83.3%, 84.8%, 92.6%, and 88.3%, respectively. The same diagnostic parameters of RT-qPCR were: 98.3%, 95.0%, 95.2%, 98.3%, and 96.7%, respectively. According to the binary logistic regression analysis, RT-qPCR (OR: 19,924, p < 0.001) was identified as the more optimal test. Conclusion: RT-qPCR targeting the 85B gene of M. tuberculosis seems to be a more useful and rapid technique than DNA-based methods for detecting live M. tuberculosis bacilli from sputum specimens. (C) 2018 The Authors. Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences.en_US
dc.description.sponsorshipIstanbul University Research Fund [BEK-2016-20201]en_US
dc.description.sponsorshipThis research was presented as a poster in the 17th Annual International Congress On Infectious Diseases (ICID) in Hyderabad, India, 2016. The poster presentation was supported financially by the Istanbul University Research Fund from project BEK-2016-20201. We also thank Prof. Dr. Nuri Kiraz (Cerrahpasa Medical Faculty, Istanbul, Turkey) and Esad Bonabi (Aydin University, Turkey) for their technical assistance related to the poster presentation.en_US
dc.identifier.doi10.1016/j.jiph.2018.02.002
dc.identifier.endpage666en_US
dc.identifier.issn1876-0341
dc.identifier.issn1876-035X
dc.identifier.issue5en_US
dc.identifier.pmid29526443en_US
dc.identifier.scopus2-s2.0-85042923176en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage662en_US
dc.identifier.urihttps://doi.org/10.1016/j.jiph.2018.02.002
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4195
dc.identifier.volume11en_US
dc.identifier.wosWOS:000442781000012en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Londonen_US
dc.relation.ispartofJournal Of Infection And Public Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject85B mRNAen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectRT-qPCRen_US
dc.subjectBACTEC MGIT 960en_US
dc.titleDiagnostic performance of the RT-qPCR method targeting 85B mRNA in the diagnosis of pulmonary Mycobacterium tuberculosis infectionen_US
dc.typeArticleen_US

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