The effects of retinoic acid on mmp-2 production, proliferation and ultrastructural morphology in rat uterus

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dc.contributor.authorKotil, Tugba
dc.contributor.authorSenturk, Sengul
dc.contributor.authorSarioglu, Turkan
dc.contributor.authorTapul, Leyla
dc.date.accessioned2024-03-13T10:34:56Z
dc.date.available2024-03-13T10:34:56Z
dc.date.issued2017
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractAims: Retinoic acid (RA) has a vital importance in order to ensure continuity and morphology in many tissues. Matrix metalloproteinases (MMPs) have significant roles in proliferation, the formation of cancers, and metastasis. In this study the effects of RA on MMP-2 production in cells of rat uterus were investigated. Methods: Twenty-four adult Sprague Dawley rats were divided into two groups, the experimental group was treated with 40 mg/kg/day 13-cis RA for 5 days by gavage. Uterine tissue sections were treated with BrdU and MMP-2 antibodies, evaluated using light microscopy. Tissues were fixed with 2.5% glutaraldehyde and evaluated using transmission electron miroscopy. Results: MMP-2 immunoreactivity decreased in the stromal cells compared with the control group and no staining of MMP-2 was observed in glandular epithelium in the experimental group. BrDU labeling of cells showed significant decrease in RA-treated group versus control group cells. Based on the electron microscopy evaluation, the surface epithelial cells of the experimental group showed vacuolization, and an accumulation of lipofuscin bodies was also observed in the gland epithelium. Cells involving autophagic vacuoles contained excess lipid granules in the entire uterus layers especially localized at the border of the endometrium and myometrium. Conclusion: RA had negative effects on cell proliferation and cell morphology and inhibited MMP-2 expression.en_US
dc.identifier.doi10.1016/j.etp.2017.06.002
dc.identifier.endpage648en_US
dc.identifier.issn0940-2993
dc.identifier.issn1618-1433
dc.identifier.issue8en_US
dc.identifier.pmid28648614en_US
dc.identifier.scopus2-s2.0-85021111837en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage643en_US
dc.identifier.urihttps://doi.org/10.1016/j.etp.2017.06.002
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4149
dc.identifier.volume69en_US
dc.identifier.wosWOS:000412261400013en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofExperimental And Toxicologic Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRetinoic aciden_US
dc.subjectUterusen_US
dc.subjectMatrix metalloproteinaseen_US
dc.subjectUltrastructureen_US
dc.subjectImmunohistochemistryen_US
dc.titleThe effects of retinoic acid on mmp-2 production, proliferation and ultrastructural morphology in rat uterusen_US
dc.typeArticleen_US

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