Effect of napabucasin and doxorubicin via the Jak2/Stat3 signaling pathway in suppressing the proliferation of neuroblastoma cells

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dc.authoridTuncer, Mehmet Cudi/0000-0001-7317-5467
dc.authoridunlu, ilker/0000-0003-4320-8381
dc.contributor.authorUnlu, Ilker
dc.contributor.authorTuncer, Mehmet Cudi
dc.contributor.authorOzdemir, Ilhan
dc.date.accessioned2025-03-09T10:48:40Z
dc.date.available2025-03-09T10:48:40Z
dc.date.issued2024
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractPurpose: Napabucasin (NP) is a natural compound that can suppress cancer cell proliferation and cell cycle by inhibition of the signal transducer and activator of transcription 3 (STAT3) gene. We examined the effects of NP on the proliferation and invasion of neuroblastoma cells (SH-SY5Y). Methods: Human neuroblastoma SH-SY5Y cell line was used in this study. NP was administered to groups at the doses of 0.3-1 mu M. Cell viability was analyzed by MTT assay. Real-time quantitative reverse transcription polymerase chain reaction and western blot analysis assessed the expressions of interleukin (IL)-6 dependent Jak2/Stat3 signaling pathway. The MTT cell viability method was applied to determine the antagonistic-synergistic effects and inhibitory concentration (IC50) doses of doxorubicin (DX) and NP. Results: It was determined that 0.3-1 mu M doses of NP killed the cells almost completely after 48 hours, and also that Jak2/Stat3 expressions decreased dose-dependently via IL-6. At the protein level, NP and DX were found to reduce Jak2 and Stat3 levels. Conclusion: NP showed that it suppresses the proliferation of neuroblastoma cells. Due to its inhibitory effect on Jak2 and Stat3, it can be used to prevent invasion of SH-SY5Y cells. NP, which can inactivate Jak2/Stat3, can be used as a treatment agent by combining with DX in proliferation pathway in neuroblastoma.
dc.identifier.doi10.1590/acb396624
dc.identifier.issn0102-8650
dc.identifier.issn1678-2674
dc.identifier.pmid39356934
dc.identifier.scopus2-s2.0-85205526836
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1590/acb396624
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4639
dc.identifier.volume39
dc.identifier.wosWOS:001328952300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherActa Cirurgica Brasileira
dc.relation.ispartofActa Cirurgica Brasileira
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250310
dc.subjectSTAT3 Transcription Factor
dc.subjectCell Culture Techniques
dc.subjectCell Proliferation
dc.subjectNeoplasms
dc.subjectBrain
dc.titleEffect of napabucasin and doxorubicin via the Jak2/Stat3 signaling pathway in suppressing the proliferation of neuroblastoma cells
dc.typeArticle

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