Cynarine Exhibits Antiproliferative Activity and Bcl-2-Mediated Apoptotic Cell Death in Breast Cancer Cells

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dc.authoridYildirim, Isil/0000-0002-3027-6054
dc.contributor.authorOzdem, Berna
dc.contributor.authorYildirim, Isil
dc.contributor.authorCetin, Ayten Kilincli
dc.contributor.authorTekedereli, Ibrahim
dc.date.accessioned2025-03-09T10:49:05Z
dc.date.available2025-03-09T10:49:05Z
dc.date.issued2024
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractCynarine is a biologically active compound that is a derivative of hydroxycinnamic acid and can exhibit a structure-function relationship and therapeutic potential due to its bioactive properties. This study aimed to investigate the antiproliferative and apoptosis activity of the Cynarine compound in breast cancer cells in vitro. MDA-MB-231 MCF7 breast cancer cells and MCF 10A non-cancer cells were used. The MTT method was used to evaluate cell viability. Acolony-forming assay was employed on the cells treated with cynarine to assess their colony-forming capability. Western blotting and annexin-propidium iodide methods with flow cytometry were used to clarify its role in apoptosis. Cell proliferation and colony-forming abilities were reduced in cynarine-treated cells at 56.2%, 67, and 3% in MCF7 and MDA-MB 231, respectively. Apoptotic cell rates after 72 hours of cynarine treatment were found at 7.15%, 42.94%, and 68.67% in MCF 10A, MCF7, and MDA-MB-231 cell lines, respectively. Apoptosis was significantly different in MDA-MB-231 and MCF7 cells treated with cynarine (p < 0.05) It has been concluded that cynarine can be used as a combination therapeutic or a precursor compound to develop new drug combinations to treat breast cancer.
dc.identifier.doi10.1007/s11094-024-03229-4
dc.identifier.endpage967
dc.identifier.issn0091-150X
dc.identifier.issn1573-9031
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85207307598
dc.identifier.scopusqualityQ4
dc.identifier.startpage963
dc.identifier.urihttps://doi.org/10.1007/s11094-024-03229-4
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4721
dc.identifier.volume58
dc.identifier.wosWOS:001338528700002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPharmaceutical Chemistry Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250310
dc.subjectcynarine
dc.subjectapoptosis
dc.subjectbreast cancer
dc.subjectflow cytometry
dc.subjectWestern blot
dc.titleCynarine Exhibits Antiproliferative Activity and Bcl-2-Mediated Apoptotic Cell Death in Breast Cancer Cells
dc.typeArticle

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