Elevated expression levels of COX-2, IL-8 and VEGF in colon adenocarcinoma

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dc.contributor.authorUslukaya, Omer
dc.contributor.authorYegin, Zeynep
dc.contributor.authorTaskesen, Fatih
dc.contributor.authorYildirim, Ibrahim Halil
dc.date.accessioned2024-03-13T10:33:05Z
dc.date.available2024-03-13T10:33:05Z
dc.date.issued2023
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractThere is growing evidence of a connection between inflammation and tumor development and NF-kappa B is an important transcription factor in the inflammation pathway. Genetic approaches have proven the role of NF-kappa B responsive genes in tumorigenesis. The NF-kappa B responsive genes products such as IL-8, VEGF and COX-2 are the key components of angiogenesis. MMP-2 and MMP-9 are playing important roles in the disruption of the extracellular matrix that may contribute to the metastasis of tumor cells. This study aimed to investigate gene expression levels of COX-2, IL-8, VEGF, MMP-2 and MMP-9 in colon tumors. A total of 34 fresh colon carcinoma specimens and paired normal adjacent tissues (NAT) were collected during the surgery and RNA isolations were carried out from specimens. Synthesis of cDNA was carried out from these RNAs with oligo dT18 primers. The transcribed cDNA was used for PCR amplification reactions for the investigated genes with beta-actin being the internal reference via the semi-quantitative RT-PCR method. A statistically significant difference was observed for COX-2, IL-8 and VEGF which were all upregulated in colon tumors compared with adjacent normal tissues (p<0.05). However, MMP-2 and MMP-9 expression levels did not change between tumor and normal tissues (p>0.05). Upregulated expression levels of COX-2, IL-8 and VEGF might occur in the early stages of tumorigenesis and detection of these mRNA levels may be beneficial for early diagnosis and management of colon tumors.en_US
dc.description.sponsorshipDicle University Scientific Research Projects Coordination Unit; [11-VF-76]en_US
dc.description.sponsorshipThis work was supported by Dicle University Scientific Research Projects Coordination Unit. Project Number: 11-VF-76.en_US
dc.identifier.doi10.14715/cmb/2023.69.6.22
dc.identifier.endpage150en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue6en_US
dc.identifier.pmid37605577en_US
dc.identifier.scopus2-s2.0-85168498482
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage146en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2023.69.6.22
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3746
dc.identifier.volume69en_US
dc.identifier.wosWOS:001059104800022
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular And Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectColon canceren_US
dc.subjectCOX-2en_US
dc.subjectIL-8en_US
dc.subjectVEGFen_US
dc.subjectMMP-2en_US
dc.subjectMMP-9en_US
dc.titleElevated expression levels of COX-2, IL-8 and VEGF in colon adenocarcinomaen_US
dc.typeArticleen_US

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