Mesenchymal stem cell-derived conditioned medium and Methysergide give rise to crosstalk inhibition of 5-HT2A and 5-HT7 receptors in neuroblastoma cells

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dc.contributor.authorSalkin, Hasan
dc.contributor.authorSatir-Basaran, Guzide
dc.contributor.authorKorkmaz, Seyda
dc.contributor.authorGonen, Zeynep Burcin
dc.contributor.authorBasaran, Kemal Erdem
dc.date.accessioned2024-03-13T10:30:56Z
dc.date.available2024-03-13T10:30:56Z
dc.date.issued2023
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractObjective: (s): We aimed to investigate the effects of mesenchymal stem cell secretome and methysergide com-bination on 5-hydroxytryptamine 2A, (5-HT2AR), 5-hydroxytryptamine 7 (5-HT7R), adenosine 2A (A2AR) re-ceptors and CD73 on neuroblastoma cell line and how they affect biological characteristics. Methysergide was used as a serotonin antagonist on the neuroblastoma cells.Materials and methods: Human dental pulp-derived stem cells (hDPSCs) used to obtain conditioned medium (CM). Methysergide drug was prepared in CM and applied to neuroblastoma cells. Analysis of 5-HT7R, 5-HT2AR, A2AR and CD73 expressions was performed by western blot and immunofluorescence staining. Total apoptosis, mitochondrial membrane depolarization, Ki-67 proliferation test, viability analysis, DNA damage and cell cycle analysis were performed in accordance with the product procedure by using biological activity test kits.Results: Our results showed that neuroblastoma cancer cells are normally on the Gs signaling axis via the sero-tonin 7 receptor and the adenosine 2A receptor. CM and Methysergide inhibited the 5-HT7 and A2A receptor levels in neuroblastoma cells. We found that CM and methysergide formed crosstalk inhibition between 5-HT2AR, 5-HT7R, A2AR and CD73. CM and Methysergide increased the total apoptosis in neuroblastoma cells and induced the mitochondrial membrane depolarization. CM and Methysergide induced the DNA damage and arrested in G0/G1 phase of cell cycle of the neuroblastoma cells.Conclusion: These findings suggest that the combination of CM and methysergite may exert a therapeutic effect on neuroblastoma cancer cells, and future in vivo studies may be important in area of neuroblastoma research to support the findings.en_US
dc.identifier.doi10.1016/j.brainres.2023.148354
dc.identifier.issn0006-8993
dc.identifier.issn1872-6240
dc.identifier.pmid36997105en_US
dc.identifier.scopus2-s2.0-85151447049en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.brainres.2023.148354
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3617
dc.identifier.volume1808en_US
dc.identifier.wosWOS:000972407500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofBrain Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMethysergideen_US
dc.subjectSerotonin receptorsen_US
dc.subjectA2A receptoren_US
dc.subjectCD73en_US
dc.subjectNeuroblastomaen_US
dc.subjectMesenchymal stem cellen_US
dc.titleMesenchymal stem cell-derived conditioned medium and Methysergide give rise to crosstalk inhibition of 5-HT2A and 5-HT7 receptors in neuroblastoma cellsen_US
dc.typeArticleen_US

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