Analysis of Virulence Factors and Antimicrobial Resistance in Salmonella Using Molecular Techniques and Identification of Clonal Relationships Among the Strains

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dc.contributor.authorUnlu, Ozge
dc.contributor.authorAktas, Zerrin
dc.contributor.authorTugrul, Hamdi Murat
dc.date.accessioned2024-03-13T10:35:32Z
dc.date.available2024-03-13T10:35:32Z
dc.date.issued2018
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractA total of 50 Salmonella enterica strains were isolated from clinical samples from 2009 to 2012 and analyzed for the presence of virulence genes found in SPI-1, SPI-2, and plasmids. The distribution and frequency of the antimicrobial resistance genes and plasmids were revealed, and pulsed-field gel electrophoresis (PFGE) patterns were investigated. Five genes were identified from the seven strains with resistance or intermediate resistance to ampicillin: blaSHV-1 (present in six strains), qnrS1 (present in five strains), blaTEM-1 (present in three strains), blaCTX-M-1 (present in one strain), and qnrB1 (present in one strain). One trimethoprim-sulfamethoxazole-resistant strain was positive for sulI but negative for sulII. In addition, we detected TEM-1 and qnrS1 in one strain; SHV-1 and qnrS1 in two strains; TEM-1, SHV-1, CTX-M-1, and qnrS1 in one strain; TEM-1, SHV-1, and qnrB1 in one strain; and SHV-1 and sulI genes in one strain together. Plasmid-based replicon typing assay revealed that all 50 strains carried FIIS, 13 carried I1, 1 carried I2, 4 carried P, 1 carried A/C, and 4 carried X1 replicon. PFGE was used to type 46 of the 50 strains and classify them into 22 major groups, 33 pulsotypes, and 8 major clusters. All strains carried all the virulence genes of interest on both Salmonella Pathogenicity Islands 1 and 2 and plasmids suggested high potential for pathogenicity. All antimicrobial-resistant strains contained at least one of the resistance genes of interest, confirming a phenotype-genotype association in antimicrobial resistance.en_US
dc.description.sponsorshipTrakya University Research Fund (TUBAP) [201476]en_US
dc.description.sponsorshipThis study was supported by a grant (Project No. 201476) from Trakya University Research Fund (TUBAP).en_US
dc.identifier.doi10.1089/mdr.2018.0042
dc.identifier.endpage1482en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue10en_US
dc.identifier.pmid29920160en_US
dc.identifier.scopus2-s2.0-85058379489en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1475en_US
dc.identifier.urihttps://doi.org/10.1089/mdr.2018.0042
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4476
dc.identifier.volume24en_US
dc.identifier.wosWOS:000435731900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSalmonella entericaen_US
dc.subjectgastroenteritisen_US
dc.subjectvirulence genesen_US
dc.subjectpulsed-field gel electrophoresisen_US
dc.subjectplasmid-based replicon typingen_US
dc.subjectantimicrobial susceptibilityen_US
dc.titleAnalysis of Virulence Factors and Antimicrobial Resistance in Salmonella Using Molecular Techniques and Identification of Clonal Relationships Among the Strainsen_US
dc.typeArticleen_US

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