Expression analysis of novel long non-coding RNAs for invasive ductal and invasive lobular breast carcinoma cases

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dc.contributor.authorAktan, Cagda
dc.contributor.authorKucukaslan, Ali Sahin
dc.contributor.authorTurk, Bilge Aydin
dc.contributor.authorYildirim, Isil
dc.date.accessioned2024-03-13T10:35:08Z
dc.date.available2024-03-13T10:35:08Z
dc.date.issued2023
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractAim: Long non-coding RNAs (LncRNAs) serve as important regulatory molecules of gene expression and protein functionality at multiple biological levels, and their deregulation plays a key role in tumorigenesis including in breast cancer metastasis. Therefore, in this study, we aim to compare the expression of novel lncRNAs in the landscape of invasive ductal carcinoma (IDC) and invasive lobular (ILC) carcinoma of breast.Main Methods: We have designed an in-silico approach to find the lncRNAs that regulate the breast cancer. Then, we used the clinical samples to carry out the verification of our in silico finding. In the present study, the tissues of breast cancer were deparaffinized. RNA was extracted by the TRIzole method. After synthesizing cDNA from the extracted RNA, expression levels of lncRNAs were analyzed by qPCR using primers specifically designed and validated for the targeted lncRNAs. In this study, breast biopsy materials from 41 female patients with IDC and 10 female patients with ILC were examined histopathological and expression changes of candidate lncRNAs were investigated in line with the findings. The results were analyzed using IBM SPSS Statistics 25 version.Results: The mean age of the cases was 53.78 +/- 14.96. The minimum age was 29, while the maximum age was 87. While 27 of the cases were pre-menopausal, 24 cases were post-menopausal. The number of hormone receptor-positive cases was found to be 40, 35, and 27 for ER, PR, and cerb2/neu, respectively. While the ex-pressions of LINC00501, LINC00578, LINC01209, LINC02015, LINC02584, ABCC5-AS1, PEX5L-AS2, SHANK2-AS3 and SOX2-OT showed significant differences (p < 0.05), the expressions of LINC01206, LINC01994, SHANK2-AS1, and TPRG1-AS2 showed no significant differences (p > 0.05). In addition, it was determined that the regulation of all lncRNAs could be able to involve in the development of cancer such as the NOTCH1, NFKB, and estrogen receptor signalings.Conclusion: As a result, it was thought that the discovery of novel lncRNAs might be an important player in the diagnosis, prognosis and therapeutic development of breast cancer.en_US
dc.identifier.doi10.1016/j.prp.2023.154391
dc.identifier.issn0344-0338
dc.identifier.issn1618-0631
dc.identifier.pmid36868097en_US
dc.identifier.scopus2-s2.0-85149213929en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.prp.2023.154391
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4268
dc.identifier.volume244en_US
dc.identifier.wosWOS:000972560500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofPathology Research And Practiceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLncRNAsen_US
dc.subjectInvasive Ductal Breast Carcinomaen_US
dc.subjectInvasive Lobular Breast Carcinomaen_US
dc.subjectBioinformaticsen_US
dc.subjectQPCRen_US
dc.subjectERen_US
dc.subjectEstrogen signaling pathwayen_US
dc.titleExpression analysis of novel long non-coding RNAs for invasive ductal and invasive lobular breast carcinoma casesen_US
dc.typeArticleen_US

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