Anti-Inflammatory Effect of Crude Momordica charantia L. Extract on 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Model in Rat and the Bioaccessibility of its Carotenoid Content

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dc.contributor.authorUnal, Nalan Gulsen
dc.contributor.authorKozak, Ayseguel
dc.contributor.authorKarakaya, Sibel
dc.contributor.authorOruc, Nevin
dc.contributor.authorBarutcuoglu, Burcu
dc.contributor.authorAktan, Cagdas
dc.contributor.authorSezak, Murat
dc.date.accessioned2024-03-13T10:35:32Z
dc.date.available2024-03-13T10:35:32Z
dc.date.issued2020
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractMomordica charantia L., known as bitter melon (BM), is a plant that belongs to the family Cucurbitaceae. Aims of this study are to investigate the anti-inflammatory effect of crude BM extract on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model in rat. It was also aimed to determine the content and bioaccessibility of carotenoids of BM. BM was purchased from local markets in Izmir, Turkey. Fruits of BM were lyophilized, powdered, and used in the experiment. Carotenoids were determined by high-performance liquid chromatography. To determine the bioaccessibility of beta-carotene, in vitro digestion was performed. Wistar albino rats were divided into four groups: group A (BM+TNBS), group B (BM), group C (TNBS), and group D (control). BM solution was given 300 mg/(kg center dot day) for 6 weeks orally. Colitis was induced by 0.25 mL of a solution containing 100 mg/kg 5% (w/v) TNBS in 50% ethanol (w/v) intrarectally after 6 weeks. After sacrification, macroscopic and microscopic evaluations were performed. Myeloperoxidase, cytokines levels (interleukin-17 [IL-17], TNF-alpha, and interleukin-10 [IL-10]) were measured in serum and colonic samples by ELISA test. Institutional Animal Ethics Committee approval was obtained. Total carotenoid content of BM was determined 11.7 mg/g dry weight as beta-carotene equivalents. Bioaccessibility of total carotenoids was determined as 2.1% with in vitro digestion. Pretreatment with crude BM extract significantly reduced weight loss, macroscopic, and microscopic colitis damages in colonic samples (P = .000), (P = .015), and (P = .026), respectively. Serum anti-inflammatory cytokine IL-10 increased significantly in both treatment groups (P = .000). BM is a rich source of carotenoids, but the bioaccessibility of its carotenoids is low. This study displays that BM has protective anti-inflammatory effects on TNBS-induced colitis.en_US
dc.description.sponsorshipEge University Scientific Research Projects [ID/number: 80/2017-TIP-007]en_US
dc.description.sponsorshipThis study was funded by the Ege University Scientific Research Projects (Project ID/number: 80/2017-TIP-007).en_US
dc.identifier.doi10.1089/jmf.2019.0124
dc.identifier.endpage648en_US
dc.identifier.issn1096-620X
dc.identifier.issn1557-7600
dc.identifier.issue6en_US
dc.identifier.pmid31702423en_US
dc.identifier.scopus2-s2.0-85085961666
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage641en_US
dc.identifier.urihttps://doi.org/10.1089/jmf.2019.0124
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4475
dc.identifier.volume23en_US
dc.identifier.wosWOS:000500485000001
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofJournal Of Medicinal Fooden_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectanti-inflammatoryen_US
dc.subjectcarotenoidsen_US
dc.subjectcolitisen_US
dc.subjectdigestionen_US
dc.subjectMomordica charantiaen_US
dc.subjectTNBS-induced colitisen_US
dc.titleAnti-Inflammatory Effect of Crude Momordica charantia L. Extract on 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Model in Rat and the Bioaccessibility of its Carotenoid Contenten_US
dc.typeArticleen_US

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