Comparative proteomics analysis of transforming growth factor-beta1-overexpressed human dental pulp stem cell-derived secretome on CD44-mediated fibroblast activation via canonical smad signal pathway

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dc.contributor.authorSalkin, H.
dc.contributor.authorAcar, M. B.
dc.contributor.authorGonen, Z. B.
dc.contributor.authorBasaran, K. E.
dc.contributor.authorOzcan, S.
dc.date.accessioned2024-03-13T10:35:22Z
dc.date.available2024-03-13T10:35:22Z
dc.date.issued2023
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractPurposeThe aim of this study investigates whether the secretome collected from human dental pulp stem cells (hDPSCs) transfected with transforming growth factor-beta1 (TGF-beta 1) is related to CD44 expression of fibroblasts and canonical smad signaling pathway via proteomic analyzes.Materials and MethodsIn order to obtain secretome, hDPSCs were conditioned with serum-free alpha-MEM in an incubator containing 37 degrees C, 5% CO2, and humidity for 18-24 h. Proteins in control and TGF-beta 1 secretome were analyzed by tandem mass spectrometry-based shotgun proteomic method. Bioinformatic evaluations were completed via Ingenuity Pathway Analysis (IPA, QIAGEN) software. CD44 expressions in fibroblasts were evaluated by real time-PCR, western blot, and immunofluorescent staining. The relationship of canonical smad pathway and CD44 was analyzed by western blot and LC-MS/MS. Cell cycle, proliferation and wound healing tests were performed in the secretome groups.ResultsVenn diagram was showed 174 common proteins were identified from each group. In the control secretome 140 unique proteins were identified and 66 entries were exclusive for TGF-beta 1 secretome. CD44 gene and protein expressions were increased in fibroblasts treated with TGF-beta 1 secretome. Relationship between targeted protein data showed that activation of the canonical TGF-beta 1/Smad pathway was up-regulated CD44 expression in fibroblasts. The canonical smad pathway-mediated upregulation of CD44 may increase the mitotic activity, proliferation, and wound healing potential in fibroblasts.ConclusionWhile TGF-beta 1-transfected hDPSC secretome may be a potential therapeutic candidate in regenerative connective tissue therapies as it induces fibroblast activation, anti-TGF-beta 1-based therapies would be considered in histopathological conditions such as pulmonary fibrosis or hepatic fibrosis.en_US
dc.identifier.doi10.1080/03008207.2022.2144733
dc.identifier.endpage218en_US
dc.identifier.issn0300-8207
dc.identifier.issn1607-8438
dc.identifier.issue2en_US
dc.identifier.pmid36421034en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage205en_US
dc.identifier.urihttps://doi.org/10.1080/03008207.2022.2144733
dc.identifier.urihttps://hdl.handle.net/20.500.12662/4395
dc.identifier.volume64en_US
dc.identifier.wosWOS:000889632700001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofConnective Tissue Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTransforming growth factor beta1en_US
dc.subjectCD44en_US
dc.subjectdental pulp stem cellsen_US
dc.subjectproteomicsen_US
dc.subjectfibroblast activationen_US
dc.titleComparative proteomics analysis of transforming growth factor-beta1-overexpressed human dental pulp stem cell-derived secretome on CD44-mediated fibroblast activation via canonical smad signal pathwayen_US
dc.typeArticleen_US

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