Berberine Attenuates Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage Via Modulating AMPK/Rho Pathway

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dc.contributor.authorKadirhan, Ozge Altintas
dc.contributor.authorKumas, Meltem
dc.contributor.authorKaratas, Ersin
dc.contributor.authorMutlu, Hasan Serdar
dc.contributor.authorKocyigit, Abdurrahim
dc.contributor.authorAsil, Talip
dc.date.accessioned2024-03-13T10:33:12Z
dc.date.available2024-03-13T10:33:12Z
dc.date.issued2020
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractPurpose: Our goal is to clarify the effectiveness of berberine (BBR) on cerebral vasospasm induced by subarachnoid hemorrhage. Methods: Thirty male Sprague-Dawley rats (350-400 g) were randomly allocated to five groups (sham group, SAH, BBR, SAH+BBR1 or SAH+BBR2. Experimental SAH model was induced by applying autologous blood into the cisterna magna at interval of 48 hours. To evaluate early and late effects of BBR, we allocated BBR treated groups as SAH+BBR1 and SAH+BBR2 (respectively, received BBR at a dose of 20 mg/kg 15 minutes and 6 hours after first SAH induction). Rats were sacrificed on 72-hour after the study onset. Cross-sections of basilar artery was investigated by histologically. Total antioxidant status (TAS) and total oxidant status (TOS) of brain tissue were studied by spectrophotometric assay. Oxidative stress index (OSI) was calculated. NAPPH Oxidase 4 (NOX4) enzyme levels were measured by ELISA method. Endothelial nitric oxide synthase (e-NOS), phosphorylated e-NOS (pe-NOS), AMP-activated protein kinase (AMPK), phosphorylated AMPK (pAMPK), Rho kinase and cingulin protein expressions were detected by Western blot analysis. Results: SAH+BBR1 and SAH+BBR2 groups significantly demonstrated lower OSI values, increased basilar artery cross-sectional luminal area in comparison with the SAH group. Increased Phosho-eNOS, eNOS, P-AMPK levels and Cingulin expression, decreased Nox4 and Rho-kinase levels were shown in BRB treated SAH groups relative to the SAH group. Conclusion: Berberine might be a neuroprotective agent to improve impaired cerebrovascular spasm.en_US
dc.identifier.doi10.30621/jbachs.2020.1125
dc.identifier.endpage317en_US
dc.identifier.issn2458-8938
dc.identifier.issn2564-7288
dc.identifier.issue3en_US
dc.identifier.startpage310en_US
dc.identifier.trdizinid417693en_US
dc.identifier.urihttps://doi.org/10.30621/jbachs.2020.1125
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/417693
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3829
dc.identifier.volume4en_US
dc.identifier.wosWOS:000579406500019en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherDokuz Eylul Univ Inst Health Sciencesen_US
dc.relation.ispartofJournal Of Basic And Clinical Health Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectberberineen_US
dc.subjectsubarachnoid hemorrhageen_US
dc.subjectcerebral vasospasmen_US
dc.subjectAMP-Activated Protein Kinaseen_US
dc.subjectRho kinaseen_US
dc.subjectendothelial dysfunctionen_US
dc.titleBerberine Attenuates Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage Via Modulating AMPK/Rho Pathwayen_US
dc.typeArticleen_US

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