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    Analysis of Serum Nickel, Silicium, Arsenic and Boron in Smoking Individuals
    (2021) Ercan, Alev Meltem; Karis, Denizhan; Alkan, Fatma Ateş; Uzan, Gülfıdan
    Smoking induces inflammation and oxidative stress via radical production from chemicals resulting in cardiocerebrovascular and respiratory diseases, cancers, stroke and sudden death. Elements found in tobacco plant and tobacco smoke are absorbed into blood circulation and transferred into blood and peripheral tissues. The aim of the present study is to evaluate the alterations of serum nickel (Ni), silicium (Si), arsenic (As) and boron (B) levels in smokers. The study groups were categorized as individuals who quitted smoking (Group 1; n: 35; 15 female/20 male), who were smoking (Group 2; n:35; 13 female, 22 male) and who never-smoked (Group 3; n; 40; 20 female/20 male). Biochemical parameters were analyzed in Biochemistry Laboratory of Haseki Traning and Research Hospital. Serum element levels were measured using Inductively Coupled Plasma Atomic Emission Spectroscopy in Trace Element Laboratory of Biophysics Department of Cerrahpasa Medical Faculty at Istanbul University-Cerrahpasa. ANOVA test and Pearson’s correlation tests were used for statistical analysis and p < 0.05 was evaluated as statistically significant. Serum Ni levels of Group 2 were higher than the other study groups with no significance. Group 2 had statistically higher serum Si and As levels than Group 1 and Group 3 (p < 0.01, p < 0.05, respectively). There was no statistical significance by means of serum B levels among study groups. Increased serum levels of Si and As in smokers might induce atherosclerosis via inflammation, dyslipidemia and burden oxidative stress. Besides, higher serum Ni levels of smokers might reflect its toxic effects. However, serum B was lower in smokers probably related with its consumption in biological defence mechanisms. Monitorization of serum nickel, silicium, arsenic and boron levels should be considered as biomarkers for smokers.
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    Evaluation of trace elements in essential thrombocytosis and reactive thrombocytosis
    (Elsevier Gmbh, 2022) Tekin, Tuba Ozkan; Karis, Denizhan; Alkan, Fatma Ates; Cetin, Guven; Ercan, Alev Meltem
    Background: Trace elements (TE) are vital for cellular mechanisms at biological, chemical and molecular levels. The effects of TE in diagnosis, progression and treatment of essential thrombocytosis (ET), which is one of the chronic myeloproliferative neoplasms is a rare clonal stem cell disease characterized by increased thrombocyte numbers with impaired function, have not been elucidated in detail yet. The aim of the present study was to investigate the effects of TE alterations in an ET model and the efficacy of TE in ET treatment protocol by means of a vast number of TE.Methods: Study groups were categorized as patients with ET diagnosis (ET group, n:30), patients with reactive thrombocytosis secondary to iron deficiency anemia (IDA-RT) (IDA-RT group, n:30) and healthy controls (HC group, n:30). Serum levels of copper (Cu), iron (Fe), cobalt (Co), chromium (Cr), aluminum (Al), silicon (Si), nickel (Ni), zinc (Zn), selenium (Se), manganese (Mn), boron (B) and magnesium (Mg) were analyzed utilizing inductively coupled plasma-optical emission spectrophotometer instrument (ICP-OES). Statistical analysis was evaluated using SPSS 23.0.Results: ET group had statistically higher serum levels of Co and Mg (p < 0.05), Ni and Mn (p < 0.001), and lower Si (p < 0.05) than IDA-RT group. ET group had statistically higher serum levels of Co and Mn (p < 0.05), and Ni (p < 0.001), and lower Al, Si and Se (p < 0.001) than HC group. Serum levels of Fe, Al and Se (p < 0.001), and Mg (p < 0.01), and Zn (p < 0.05) in IDA-RT group were significantly lower than HC group.Conclusion: This novel study pointed out that alterations of many serum TE by means of both increment or decrement might have close relationship with mechanisms and complications of ET onset and follow-up. We consider that further research of TE would elucidate ethiopathogenesis and prognosis of ET. Thus, analysis of serum trace elements in essential thrombocytosis patients may be an important protocol by means of diagnosis, treatment and follow-up intervals.
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    Oral Microbiota Signatures in the Pathogenesis of Euthyroid Hashimoto's Thyroiditis
    (Mdpi, 2023) Erdem, Mustafa Genco; Unlu, Ozge; Ates, Fatma; Karis, Denizhan; Demirci, Mehmet
    One of the most prevalent autoimmune illnesses in the world is Hashimoto's thyroiditis, whose pathogenesis is still unknown. The gut-thyroid axis is frequently examined, and although oral health affects thyroid functions, there are limited data on how oral microbiota is linked to Hashimoto's thyroiditis. The study aims to identify the oral microbiota from saliva samples taken from treated (with levothyroxine) and untreated female euthyroid Hashimoto's thyroiditis patients as well as healthy controls who were age- and sex-matched to compare the oral microbiota across the groups and to contribute preliminary data to the literature. This study was designed as a single-center cross-sectional observational study. Sixty (60) female patients with euthyroid Hashimoto's thyroiditis (HT) and eighteen (18) age- and gender-matched healthy controls were included in this study. Unstimulated saliva samples were collected. After DNA isolation, sequencing was performed by targeting the V3-V4 gene regions of the 16S rRNA on the MiSeq instrument. R scripts and SPSS were used for bioinformatic and statistical analysis. No significant differences were found in the diversity indices. However, Patescibacteria phylum showed a significantly higher abundance (3.59 vs. 1.12; p = 0.022) in the oral microbiota of HT patients compared to HC. In the oral microbiota, the euthyroid HT group had approximately 7, 9, and 10-fold higher levels of the Gemella, Enterococcus, and Bacillus genera levels than healthy controls, respectively. In conclusion, the results of our study demonstrated that Hashimoto's thyroiditis causes changes in the oral microbiota, whereas the medicine used to treat the condition had no such effects. Therefore, revealing the core oral microbiota and long-term follow-up of the HT process by conducting extensive and multicenter studies might provide some important data for understanding the pathogenesis of the disease.