Determination of Biotin Interference in Pediatric Obesity Related ELISA Research Kits Biotin Interference in Manual ELISA Kits

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dc.contributor.authorKürkçü Kahraman, Ezgi
dc.contributor.authorDonma, Orkide
dc.contributor.authorDonma, Mustafa Metin
dc.contributor.authorYilmaz, Ahsen
dc.contributor.authorGüzel, Savaş
dc.date.accessioned2026-01-31T15:04:21Z
dc.date.available2026-01-31T15:04:21Z
dc.date.issued2025
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractBackground: Although high-dose biotin interference in automated immunoassays is now considered, there are very few studies showing biotin interference in manually operated research kits, especially with enzyme-linked immunosorbent assay (ELISA). The aims of our study were to determine the effects of biotin interference on various parameters, including leptin, leptin receptor (LEPR), ghrelin, acylated ghrelin, deacylated ghrelin, ghrelin receptor (GHSR), kisspeptin (KISS1), kisspeptin receptor (KISS1R), preptin, peroxisome proliferator activated receptor gamma (PPAR?), nod-like receptor pyrin domain-containing 3 (NLRP3) and interleukin-18 (IL-18), which contribute to energy homeostasis in healthy and obese children. Methods: Serum pools were prepared from healthy and obese individuals, and biotin concentrations in samples containing different amounts of biotin were measured via sandwich and competitive ELISA methods. In addition, possible biotin interactions were investigated by determining the concentrations of all the study parameters in serum pools containing different amounts of biotin. Results: We found that the biotin-competitive, ghrelin-competitive, KISS1-competitive, GHSR, leptin and LEPR ELISA kits were less affected by biotin interference and the results of these assay kits were more reliable. Unexpectedly, high levels were also measured in the biotin sandwich ELISA kit, indicating that biotin interference can also occur in manually operated assay kits. Conclusions: Biotin exhibited an interference effect even in well-functioning, qualified kits, and this negative effect was less common in competitive kits. Biotin interference was closely associated with the quality of the research kit, the parameters studied, and the presence of high biotin concentrations in the blood. © 2025 The Author(s). Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.
dc.description.sponsorshipIstanbul Üniversitesi-Cerrahpasa, IUC, (34574)
dc.identifier.doi10.1002/jcla.70079
dc.identifier.issn0887-8013
dc.identifier.issue16
dc.identifier.pmid40657850
dc.identifier.scopus2-s2.0-105010696586
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/jcla.70079
dc.identifier.urihttps://hdl.handle.net/20.500.12662/10503
dc.identifier.volume39
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherJohn Wiley and Sons Inc
dc.relation.ispartofJournal of Clinical Laboratory Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20260128
dc.subjectbiotin interference
dc.subjectELISA
dc.subjectpediatric obesity
dc.titleDetermination of Biotin Interference in Pediatric Obesity Related ELISA Research Kits Biotin Interference in Manual ELISA Kits
dc.typeArticle

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