Regeneration of Bellevalia pseudolongipes Karabacak & Yıldırım Under in Vitro Conditions

Basit Öğe Kaydı
dc.contributor.authorGEZER, Rukiye
dc.contributor.authorTürkoglu, Nalan
dc.contributor.authorKAHRIZ, PARISA POURALI
dc.contributor.authorKahriz, Mahsa Pourali
dc.date.accessioned2026-01-31T15:02:29Z
dc.date.available2026-01-31T15:02:29Z
dc.date.issued2025
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractProfitable viable in vitro, multiplication of endemic bulbous geophyte Bellevalia pseudolongipes is challenging and is desired for commercial exploitation and conservation. This study aimed to multiply this plant species using double-scale explants using 1/4 (0.5 cm), 1/6 (0.33 cm), or 1/8 th (0.25 cm) longitudinally sliced bulb scale tissues lying in the 1st, and 2nd, clusters of B. Pseudolongipes mother bulbs. These were cultured on MS; White and Gamborg B5 media enriched with 1.11 ?M - 5.44 ?M BAP and 1.34 ?M NAA (5 combinations) to compare the regeneration potential of fresh and 2-month stored bulb scale explants. Bulblet induction was observed on all concentrations of BAP-NAA on all culture media. MS medium modified with 1.11 ?M BAP and 1.34 ?M NAA induced maximum number of 3.56 bulblets explant on 0.25 cm wide two scale explants. The regeneration capability of White and Gamborg B5 medium was less compared to bulblets induced on MS medium. The biggest bulbs were observed on Gamborg B5 medium, which differed significantly from the bulblets induced on White and MS media. Treatment of regenerated bulblets with sucrose improved bulblet diameter by ~2 mm. The bulblet diameter varied between 0.42-0.83 cm. The bulblets were rooted on MS medium without using any auxin. Furthermore, the study revealed the potential of 2 scale explants from the outer cluster for multiplication and laid the groundwork for future breeding and tissue culture-based multiplication to conserve the species. The experimental results provide a useful reference for the efficacy of bulb scales for regeneration.
dc.identifier.doi10.5281/zenodo.15781264
dc.identifier.endpage654
dc.identifier.issn2717-7238
dc.identifier.issue3
dc.identifier.startpage644
dc.identifier.trdizinid1339851
dc.identifier.urihttps://doi.org/10.5281/zenodo.15781264
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1339851
dc.identifier.urihttps://hdl.handle.net/20.500.12662/10458
dc.identifier.volume9
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofISPEC journal of agricultural sciences (Online)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20260128
dc.subjectEndemic species
dc.subjectgenotypes
dc.subjectpropagation
dc.subjectSouth Eastern Anatolia
dc.subjectbulb scales
dc.subjectsurface disinfection
dc.titleRegeneration of Bellevalia pseudolongipes Karabacak & Yıldırım Under in Vitro Conditions
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu