Comparison of different final irrigant agitation techniques for the removal of enterococcus faecalis biofilms from root canals: An in vitro study [Comparação de diferentes técnicas finais de agitação de irrigantes para a remoção de biofilmes de enterococcus faecalis de canais radiculares: Estudo in vitro]

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dc.contributor.authorGunec H.G.
dc.contributor.authorHaznedaroglu F.
dc.contributor.authorKulekci G.
dc.contributor.authorTopcuoglu N.B.
dc.contributor.authorÖzcan M.
dc.date.accessioned2024-03-13T10:01:13Z
dc.date.available2024-03-13T10:01:13Z
dc.date.issued2018
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractObjective: The aim of this in vitro study was to compare the effectiveness of different final irrigant agitation techniques in the removal of Enterococcus faecalis biofilms from root canals. Material and Methods: In total, the root canals of 85 extracted single-rooted human maxillary incisors teeth were prepared using the Revo-S system to a 40/06 size. The apical foramen of each tooth was sealed by light-cured resin composite material to obstruct bacterial leakage. The specimens were sterilized in an autoclave at 121°C for 15 min and stored until further use. All teeth except five (negative control group) were inoculated with Enterococcus faecalis and incubated in a CO2 chamber at 37°C for 7 days; the trypticase soy broth was changed every 2 days. For the determination of possible biofilm formation, five of the 80 teeth were randomly selected as a positive control group; one tooth of positive control group was analysed for biofilm development by scanning electron microscope (SEM) and these teeth received no final irrigant agitation procedure. Then, the remaining 75 teeth were randomly divided into five test groups (n=15 each) and were sequentially irrigated with 5% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid and 5% NaOCl. Following each irrigant application, different final irrigant agitation techniques were introduced for 60 s (3×20-s sessions). Group 1 received manual– dynamic agitation, group 2 received passive ultrasonic agitation (PUI), group 3 received EndoActivator agitation, group 4 received photon-initiated photoacoustic streaming (PIPS) with the Er:YAG laser and group 5 received conventional syringe irrigation. Colony-forming units (CFUs) were counted in samples from the positive control and test groups. Data were analysed using Kruskal– Wallis and post-hoc Mann–Whitney U multiple comparison tests. Results: E. faecalis elimination was significantly better in the experimental groups than in the positive control groups (p < 0.001). Manual–dynamic agitation and conventional syringe irrigation, with no significant differences between the two groups. Conclusion: Essentially, CFU reduction was significantly greater in the PUI, EndoActivator and PIPS groups than in the manual–dynamic agitation and conventional syringe irrigation groups (p <0.001), with no significant differences among the former three groups. © 2018, Universidade Estadual Paulista, Institute of Science and Technology of Sao Jose dos Campos. All rights reserved.en_US
dc.identifier.doi10.14295/bds.2018.v21i4.1599
dc.identifier.endpage394en_US
dc.identifier.issn2178-6011
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85057219714en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage386en_US
dc.identifier.urihttps://doi.org/10.14295/bds.2018.v21i4.1599
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3051
dc.identifier.volume21en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniversidade Estadual Paulista, Institute of Science and Technology of Sao Jose dos Camposen_US
dc.relation.ispartofBrazilian Dental Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEnterococcus faecalisen_US
dc.subjectIrrigant agitationen_US
dc.subjectIrrigant solutionsen_US
dc.subjectPassive ultrasonic irrigationen_US
dc.subjectPhoton-initiated photoacoustic streamingen_US
dc.titleComparison of different final irrigant agitation techniques for the removal of enterococcus faecalis biofilms from root canals: An in vitro study [Comparação de diferentes técnicas finais de agitação de irrigantes para a remoção de biofilmes de enterococcus faecalis de canais radiculares: Estudo in vitro]en_US
dc.typeArticleen_US

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