Investigation of the antitumoral activity of tarantula cubensis extract on human hepatocelular cancer cells

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dc.contributor.authorKurtkulağı, Özgür
dc.contributor.authorcatal, oguz
dc.contributor.authorŞeyda Karabörk
dc.contributor.authorKARA, Sinem
dc.contributor.authorÇETINKAYA, AYHAN
dc.contributor.authorsit, mustafa
dc.contributor.authorÖzer, Bahri
dc.date.accessioned2026-01-31T15:02:21Z
dc.date.available2026-01-31T15:02:21Z
dc.date.issued2025
dc.departmentİstanbul Beykent Üniversitesi
dc.description.abstractAim: To investigate the antitumor effects of tarantula cubensis alcoholic extract (TCE) on the human hepatocellular cell line (HepG2). Methods: Various concentrations (1, 5, 10, 20, 50, 100, 250, and 500 ?g/ml) of TCE were applied to HepG2 cells, which were then incubated for 24, 48, and 72 hours. Analysis was performed using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Immunohistochemistry and apoptosis (Ki-67) analysis were conducted on cells treated with 1 and 5 ?g/ml TCE for 24, 48, and 72 hours. Results: The most appropriate doses of TCE for the HepG2 cell line were found to be 5 ?g/ml at 24 hours and 1 ?g/ml at 48 and 72 hours. Ki-67 H-SCOR in HepG2 cells treated with TCE significantly decreased compared to the control group (p<0.001). Conclusion Our study suggests that TCE may alter normal cancer physiology by stimulating apoptosis in HepG2 cells via the Ki-67 pathway, leading to cell death. Therefore, TCE has the potential to provide a new perspective in the treatment of HCC.
dc.identifier.doi10.30714/j-ebr.2024.230
dc.identifier.endpage23
dc.identifier.issn2618-6454
dc.identifier.issue1
dc.identifier.startpage14
dc.identifier.trdizinid1348818
dc.identifier.urihttps://doi.org/10.30714/j-ebr.2024.230
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1348818
dc.identifier.urihttps://hdl.handle.net/20.500.12662/10412
dc.identifier.volume8
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofExperimental Biomedical Research
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20260128
dc.subjectApoptosis
dc.subjectHepG2
dc.subjectKi-67
dc.subjecthepatocelluler cancer
dc.subjecttarantula cubensis alcholic extract
dc.subjecttheranekron.
dc.titleInvestigation of the antitumoral activity of tarantula cubensis extract on human hepatocelular cancer cells
dc.typeArticle

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