Identification of Methicillin resistant Staphylococcus aureus in yoghurts from Şanliurfa by real-time PCR [Sanliurfa'da Yoğurtlarda Metisilin Dirençli Staphylococcus aureus (MRSA) Varliğinin Real-Time PCR ile Belirlenmesi]

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dc.contributor.authorDemirci M.
dc.contributor.authorYiğin A.
dc.contributor.authorKiliç Altun S.
dc.contributor.authorDinç H.
dc.contributor.authorErol S.
dc.date.accessioned2024-03-13T10:01:24Z
dc.date.available2024-03-13T10:01:24Z
dc.date.issued2017
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractMethicillin resistant Staphylococcus aureus (MRSA) is one of the most important pathogenic microorganisms that display multiple drug resistance, causing severe infections in animals and humans. Contaminated or raw milk products, such as yoghurt, are one of the root causes of such MRSA infections for the consumers. Recent studies on clonal investigation revealed the same clonal isolates of animals in human infections. Thus, the epidemiology of these bacteria in food products and animals has gained importance. Therefore, the aim of this study was to epidemiologically detect MRSA in yoghurt samples from Urfa via real-time PCR. To detect MRSA via real-time PCR, the mecA gene was investigated on the DNA obtained from the yoghurt samples. Among 64 yoghurt samples, of which 55 were unpackaged and 9 were packaged, the MRSA mecA gene was only detected in 2 (3.13 %) unpackaged samples via real-time PCR. These results showed that it was possible to detect and epidemiologically investigate MRSA, an important pathogen for human and animal health, via real-time PCR from yoghurt samples with direct DNA isolation. In addition, this pathogen can be found in the products sold in Sanliurfa, which indicates that it may lead to severe diseases in case of consumption, if not conformed to hygienic conditions.en_US
dc.identifier.endpage6en_US
dc.identifier.issn1307-1130
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85039560092en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage4en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12662/3190
dc.identifier.volume11en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherSelcuk Universityen_US
dc.relation.ispartofJournal of Applied Biological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectContaminationen_US
dc.subjectMRSAen_US
dc.subjectReal-time PCRen_US
dc.subjectYoghurten_US
dc.titleIdentification of Methicillin resistant Staphylococcus aureus in yoghurts from Şanliurfa by real-time PCR [Sanliurfa'da Yoğurtlarda Metisilin Dirençli Staphylococcus aureus (MRSA) Varliğinin Real-Time PCR ile Belirlenmesi]en_US
dc.typeArticleen_US

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